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Recovery of large preantral follicles from buffalo ovary: Effect of season and corpus luteum.

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Title Recovery of large preantral follicles from buffalo ovary: Effect of season and corpus luteum.
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Creator Gupta PSP
Ramesh HS
Nandi S
Ravindra JP
 
Subject Preantral follicle
Corpus luteum
Season
Buffalo
 
Description Not Available
Preantral follicle can be considered as an alternative source of oocyte for in vitro production of embryos. The objective of the present study was to standardize a procedure for the isolation of large preantral follicles (>150-500 micro m) from buffalo ovaries and to determine the effect of season and the presence of corpus luteum on the recovery rate of the large preantral follicles. A combined enzymatic cum mechanical approach was adopted to recover the large preantral follicles. In the first experiment, the ovarian cortical pieces were suspended in trypsin (1000-1500 BAEE units for milligrams of solid) and incubated at various temperatures for different periods, i.e. (1) trypsin (1%), 37 degrees C for 10 min; (2) trypsin (1%), 37 degrees C for 10 min + 4 degrees C for 3 h; (3) trypsin (0.5%), 37 degrees C for 20 min; (4) trypsin (0.25%), 37 degrees C for 20 min. Although there was no significant difference (P>0.05) among the different protocols, the first protocol yielded more follicles (3.2, 2.6, 1.8 and 1.5 per ovary, respectively). Hence, the first protocol was selected and used in the second and third experiments. In the second experiment, the effect of season, i.e. peak breeding season (October-March) versus low breeding season (April-September) was evaluated on the recovery rate of the large preantral follicles. The recovery rate of large preantral follicles from the ovaries during the peak breeding season was significantly (P
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Date 2021-08-03T04:26:08Z
2021-08-03T04:26:08Z
2007-09-01
 
Type Journal
 
Identifier Gupta PSP, Ramesh HS, Nandi S and Ravindra JP (2007) Recovery of large preantral follicles from buffalo ovary: Effect of season and corpus luteum. Animal Reproduction Science. 101: 145- 152.
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http://krishi.icar.gov.in/jspui/handle/123456789/52409
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER