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In vitro embryo development and blastocyst hatching rates following vitrification of river buffalo embryos produced from oocytes recovered from slaughterhouse ovaries or live animals by ovum pick-up

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Title In vitro embryo development and blastocyst hatching rates following vitrification of river buffalo embryos produced from oocytes recovered from slaughterhouse ovaries or live animals by ovum pick-up
 
Creator Manjunatha BM
PSP Gupta
JP Ravindra
M Devaraj
S Nandi
 
Subject OPU
Vitrification
Oocyte developmental competence
Buffalo
 
Description Not Available
The present study was undertaken to determine whether the source of oocytes (ovum pick up versus slaughterhouse ovaries) affected in vitro embryo production and embryo survival (as measured by blastocyst hatching rates) following vitrification in buffaloes (Bubalus bubalis). Oocytes recovered from live buffaloes (n=6) by ovum pick up (OPU) and by manual aspiration from slaughterhouse ovaries were in vitro matured, fertilized and cultured to blastocyst stage under same culture conditions. Vitrification of blastocysts was carried out in two steps at 24 degrees C. Embryos were equilibrated in 10% EG+10% DMSO+0.3 M sucrose in base medium for 4 min. Subsequently, the embryos were transferred into 25% EG+25% DMSO+0.3 M sucrose in base medium for 45 s and then the embryos were loaded into straws and immersed in liquid nitrogen. Following warming, blastocysts were cultured in vitro for 48 h to assess hatching. Oocytes derived from live animals by OPU resulted in a significantly higher blastocyst yield then those derived from slaughterhouse ovaries (30.6+/-4.3 versus 18.5+/-1.8). Blastocyst hatching rates following vitrification of buffalo embryos produced from the oocytes collected from live animals by OPU was significantly higher than the oocytes collected from slaughterhouse ovaries (52.8+/-4.2 versus 40.2+/-4.4). In conclusion, the present study showed that source of oocytes (OPU versus slaughterhouse ovaries) affects the in vitro embryo development and blastocyst hatching rates following vitrification of embryos in buffaloes.
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Date 2021-08-03T04:29:57Z
2021-08-03T04:29:57Z
2008-03-03
 
Type Journal
 
Identifier Manjunatha BM, PSP Gupta, JP Ravindra, M Devaraj and S Nandi. (2008). In vitro embryo development and blastocyst hatching rates following vitrification of river uffalo embryos produced from oocytes recovered from slaughterhouse ovaries or live animals by ovum pick-up. Animal Reproduction Science. 104: 419426
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http://krishi.icar.gov.in/jspui/handle/123456789/52410
 
Language English
 
Relation Not Available;
 
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