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Cell culture adaptation mutations in foot-and-mouth disease virus serotype A capsid proteins: implications for receptor interactions

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Title Cell culture adaptation mutations in foot-and-mouth disease virus serotype A capsid proteins: implications for receptor interactions
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Creator Jajati K Mohapatra
Laxmi K Pandey
Devendra K Rai
Biswajit Das
Luis L Rodriguez
Manoranjan Rout
Saravanan Subramaniam
Aniket Sanyal
Elizabeth Rieder
Bramhadev Pattnaik
 
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Description Not Available
In this study we describe the adaptive changes fixed on the capsid of several foot-and-mouth disease virus serotype A strains during propagation in cell monolayers. Viruses passaged extensively in three cell lines (BHK-21, LFBK and IB-RS-2) consistently gained positively charged amino acids in the putative heparin-sulfate-binding pocket (VP2 βE-βF loop, VP1 C-terminus and VP3 β-B knob) surrounding the fivefold symmetry axis (VP1 βF-βG loop) and at other discrete sites on the capsid (VP3 βG-βH loop, VP1 C-terminus, VP2 βC strand and VP1 βG-βH loop). A lysine insertion in the VP1 βF-βG loop of two of the BHK-21-adapted viruses supports the biological advantage of positively charged residues acquired in cell culture. The charge transitions occurred irrespective of cell line, suggesting their possible role in ionic interaction with ubiquitous negatively charged cell-surface molecules such as glycosaminoglycans (GAG). This was supported by the ability of the cell-culture-adapted variants to replicate in the integrin-deficient, GAG-positive CHO-K1 cells and their superior fitness in competition assays compared with the lower passage viruses with WT genotypes. Substitutions fixed in the VP1 βG-βH loop (-3, -2 and +2 'RGD' positions) or in the structural element known to be juxtaposed against that loop (VP1 βB-βC loop) suggest their possible role in modulating the efficiency and specificity of interaction of the 'RGD' motif with αv-integrin receptors. The nature and location of the substitutions described in this study could be applied in the rapid cell culture adaptation of viral strains for vaccine production.
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Date 2021-07-30T05:47:17Z
2021-07-30T05:47:17Z
2015-03-01
 
Type Research Paper
 
Identifier Mohapatra JK, Pandey LK, Rai DK, Das B, Rodriguez LL, Rout M, Subramaniam S, Sanyal A, Rieder E, Pattnaik B. Cell culture adaptation mutations in foot-and-mouth disease virus serotype A capsid proteins: implications for receptor interactions. J Gen Virol. 2015 Mar;96(Pt 3):553-564. doi: 10.1099/vir.0.071597-0. Epub 2014 Nov 7. PMID: 25381054.
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http://krishi.icar.gov.in/jspui/handle/123456789/51410
 
Language English
 
Relation Not Available;
 
Publisher Microbiology Society