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In vitro developmental competence of buffalo oocytes collected at various stages of the estrous cycle.

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Title In vitro developmental competence of buffalo oocytes collected at various stages of the estrous cycle.
Not Available
 
Creator Manjunatha BM
PSP Gupta
JP Ravindra
M Devaraj
HS Ramesh
S Nandi
 
Subject Oocyte developmental competencet
Buffalo
follicle
Embryo
Dominan
 
Description Not Available
The objective was to determine the in vitro developmental competence of buffalo oocytes collected from battoir-derived ovaries at various stages of the estrous cycle and follicular status. In Experiment 1, ovaries (n = 476 pairs) were collected and divided into the following five groups: (a) ovaries with a corpus hemorragicum and no dominant follicle (CH-NO-DF); (b) ovaries with a mature functional corpus luteum (CL) and a dominant follicle (CL-DF); (c) ovaries with a mature functional CL and no dominant follicle (CL-NO-DF); (d) ovaries with a regressing CL and a dominant follicle (RCL-DF); and (e) ovaries without any luteal structures and only small follicles (ANEST). In Experiment 2, 144 pairs of ovaries with a CL (or regressing CL) and a dominant follicle were collected and follicles were classified as dominant, largest subordinate, and subordinate. In both experiments, the dominant follicle was defined as any follicle >10 mm in diameter that exceeded the diameter of all other (subordinate) follicles. Although oocytes were collected from each group of ovaries, only Grades A or B oocytes were used for in vitro embryo production. Cleavage rates were higher (P < 0.05) from oocytes collected from ovaries in the CH-NO-DF (59.6%) and CL-NO-DF (59.2%) groups than those collected from CL-DF (52.2%) and ANEST (43.6%) groups. The yield of transferable embryos was higher (P < 0.05) from oocytes collected from CH-NO-DF (27.4%) and CL-NO-DF (24.0%) ovaries than from CL-DF (16.2%), RCL-DF (15.4%), and lowest (P < 0.05) from ANEST (8.8%). In Experiment 2, oocytes from the dominant follicle had a higher (P < 0.05) cleavage rate (65.2 %) and transferable embryo yield (30.2%) than those collected from the largest subordinate and subordinate follicles. In conclusion, oocyte competence depended on the morphofunctional state of ovaries. Oocyte development was maximal in pairs of ovaries with a corpus hemorragicum or CL and no dominant follicle; in paired ovaries with a CL and a dominant follicle, development was maximal in oocytes derived from the dominant follicle.
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Date 2021-08-04T04:37:45Z
2021-08-04T04:37:45Z
2007-10-01
 
Type Journal
 
Identifier Manjunatha BM, PSP Gupta, JP Ravindra, M Devaraj, HS Ramesh, S Nandi (2007). In vitro developmental competence of buffalo oocytes collected at various stages of the estrous cycle. Theriogenology. 68:882888
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/52796
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER