Selection of developmentally competent buffalo oocytes by brilliant cresyl blue staining before IVM
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Title |
Selection of developmentally competent buffalo oocytes by brilliant cresyl blue staining before IVM
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Creator |
Manjunatha BM
PSP Gupta M Devaraj JP Ravindra S Nandi |
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Subject |
Brilliant cresyl blue
Buffalo Oocyte developmental competence In vitro production |
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Description |
Not Available
The brilliant cresyl blue (BCB) test determines the activity of glucose-6-phosphate dehydrogenase (G6PDH); the activity of this enzyme is greatest in growing oocytes, but it declines as oocytes mature. The objective was to develop and evaluate this test for assessing development of buffalo oocytes (to select developmentally competent oocytes for increased in vitro embryo production). Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with 0.4% BSA) for 90 min at 38.5 degrees C in a humidified air atmosphere; those with or without blue coloration of the cytoplasm were designated as BCB+ and BCB-, respectively. In Experiment 1, oocytes were exposed to 13, 26, or 39 microM BCB. There were fewer BCB+ oocytes after exposure to 13 microM BCB (10%) than after exposure to 26 or 39 microM BCB (57.2 and 61.8%; P Not Available |
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Date |
2021-08-03T04:37:07Z
2021-08-03T04:37:07Z 2007-10-24 |
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Type |
Journal
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Identifier |
Manjunatha BM, PSP Gupta, M Devaraj, JP Ravindra and S Nandi (2007) Selection of developmentally competent buffalo oocytes by brilliant cresyl blue staining before IVM. Theriogenology. 68:12991304
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/52411 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Europe PMC
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