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The biochemical composition of ovine follicular fluid in relation to follicle size.

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Title The biochemical composition of ovine follicular fluid in relation to follicle size.
Not Available
 
Creator Nandi S
V Girish Kumar
BM Manjunatha
PSP Gupta
 
Subject biochemical composition
follicular fluid
ovine
 
Description Not Available
The aim of this study was to biochemically characterize ovine follicular fluid and to relate possible changes in composition to follicular size. Ovaries were collected from adult and cycling non-pregnant slaughtered sheep (Ovis aries) during breeding season. A total of 104 pairs of ovaries were investigated and these data were then compared. Follicular fluid was aspirated from small (< 2 mm), medium (2–4 mm) and large (> 4 mm) nonatretic ovarian follicles. The follicular fluid was centrifuged at 4°C and 5000 g for 30 min to remove any cells and stored at −80°C prior to assay. Follicular fluid samples were analyzed for glucose, total protein, cholesterol, triglycerides, lactate, urea, creatinine, sodium, potassium, chloride, calcium, phosphorus, magnesium, acid phosphatase, alkaline phosphatase, and lactate dehydrogenase. Data were analyzed by the linear regression model. As follicles became larger, the concentrations of glucose and cholesterol significantly (P < 0.05) increased while those of triglycerides, lactate, alkaline phosphatase and lactate dehydrogenase significantly (P < 0.05) decreased.
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Date 2021-08-03T05:06:21Z
2021-08-03T05:06:21Z
2007-01-16
 
Type Journal
 
Identifier Nandi S, V Girish Kumar, BM Manjunatha and PSP Gupta (2007). The biochemical composition of ovine follicular fluid in relation to follicle size. Development Growth & Differentiation 49:61-66.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/52420
 
Language English
 
Relation Not Available;
 
Publisher The Japanese Societies of Developmental Biologist