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Erratum to: The carboxy-terminal half of nonstructural protein 3A is not essential for foot-and-mouth disease virus replication in cultured cell lines

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Title Erratum to: The carboxy-terminal half of nonstructural protein 3A is not essential for foot-and-mouth disease virus replication in cultured cell lines
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Creator Mrutyunjay Behura
Jajati K Mohapatra
Laxmi K Pandey
Biswajit Das
Mukesh Bhatt
Saravanan Subramaniam
Bramhadev Pattnaik
 
Subject Culture Cell Line Parental Virus Infectious cDNA Clone Bull Calf Marker Vaccine
 
Description Not Available
In foot-and-mouth disease (FMD)-endemic parts of the globe, control is mainly implemented by preventive vaccination with an inactivated purified vaccine. ELISAs detecting antibodies to the viral nonstructural proteins (NSP) distinguish FMD virus (FMDV)-infected animals in the vaccinated population (DIVA). However, residual NSPs present in the vaccines are suspected to be a cause of occasional false positive results, and therefore, an epitope-deleted negative marker vaccine strategy is considered a more logical option. In this study, employing a serotype Asia 1 FMDV infectious cDNA clone, it is demonstrated that while large deletions differing in size and location in the carboxy-terminal half of 3A downstream of the putative hydrophobic membrane-binding domain (deletion of residues 86-110, 101-149, 81-149 and 81-153) are tolerated by the virus without affecting its infectivity in cultured cell lines, deletions in the amino-terminal half (residues 5-54, 21-50, 21-80, 55-80 and 5-149) containing the dimerization and the transmembrane domains are deleterious to its multiplication. Most importantly, the virus could dispense with the entire carboxy-terminal half of 3A (residues 81-153) including the residues involved in the formation of the 3A-3B1 cleavage junction. The rescue of a replication-competent FMDV variant carrying the largest deletion ever in 3A (residues 81-153) and the fact that the deleted region contains a series of linear B-cell epitopes inspired us to devise an indirect ELISA based on a recombinant 3A carboxy-terminal fragment and to evaluate its potential to serve as a companion diagnostic assay for differential serosurveillance if the 3A-truncated virus is used as a marker vaccine.
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Date 2021-08-12T04:47:13Z
2021-08-12T04:47:13Z
2016-03-03
 
Type Research Paper
 
Identifier Behura M, Mohapatra JK, Pandey LK, Das B, Bhatt M, Subramaniam S, Pattnaik B. Erratum to: The carboxy-terminal half of nonstructural protein 3A is not essential for foot-and-mouth disease virus replication in cultured cell lines. Arch Virol. 2016 May;161(5):1307. doi: 10.1007/s00705-016-2836-5. Erratum for: Arch Virol. 2016 May;161(5):1295-305. PMID: 27038829.
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http://krishi.icar.gov.in/jspui/handle/123456789/55154
 
Language English
 
Relation Not Available;
 
Publisher SpringerLink