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Differential expression and localization of TRPV channels in the mature sperm of Anas platyrhynchos

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Title Differential expression and localization of TRPV channels in the mature sperm of Anas platyrhynchos
Not Available
 
Creator Rakesh Kumar Majhi 1,2 | Ashutosh Kumar 1,2 | Sunil C. Giri 3 | Chandan Goswami 1,2
 
Subject TRPV1, TRPV2, TRPV3, TRPV4, TRPV5, TRPV6
 
Description Not Available
Sperm cells perform precise chemotactic and thermotactic movement which is crucial
for fertilization. However, the key molecules involved in detection of different
chemical and physical stimuli which guide the sperm during navigation are not well
understood. Ca2+-signalling mediated by ion channels seem to play important role
in motility and other fertility parameters. In this work, we explored the endogenous
localization pattern of TRPV channels in the mature spermatozoa of avian species.
Using sperm from white pekin duck (Anas platyrhynchos) as the representative avian
model, we demonstrate that duck sperm endogenously express the thermosensitive
channels TRPV1, TRPV2, TRPV3, TRPV4, and highly Ca2+-selective channels TRPV5
and TRPV6 in specific yet differential locations. All of these TRPV channels are enriched
in the sperm tail, indicating their relevance in sperm motility. Interestingly, the
TRPV3 and TRPV4 channels are present in the mitochondrial region. Calcium selective
TRPV5 channel is exclusively present in sperm tail and is most abundant among
the TRPV channels. This is the first report describing the endogenous presence of
TRPV2 and TRPV3 channels in the sperm of any species. Using confocal imaging and
super-resolution imaging, we demonstrate that though the TRPV channels are evolutionarily
closely related, they have distinct localization pattern in the duck sperm,
which could impact their role in fertilization.
Intramural funding to NISER (Department of Atomic Energy, Govt. of India), Grant/Award Number: 0000
 
Date 2021-08-16T06:43:05Z
2021-08-16T06:43:05Z
2020-10-01
 
Type Article
 
Identifier Rakesh Kumar Majhi, Ashutosh Kumar , Sunil C. Giri, Chandan Goswami (2020) Differential expression and localization of TRPV channels in the mature sperm of Anas platyrhynchos. Reproduction in Domestic Animals 55(11), 1619-1628
0936-6768
http://krishi.icar.gov.in/jspui/handle/123456789/57202
 
Language English
 
Relation Not Available;
 
Publisher WILEY-VCH-GmBH