Evaluation of ELISA and PCR in parallel to single intradermal cervical tuberculin test (SICT) for diagnosis of tuberculosis in buffaloes
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Title |
Evaluation of ELISA and PCR in parallel to single intradermal cervical tuberculin test (SICT) for diagnosis of tuberculosis in buffaloes
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Creator |
Bincy Joseph, Amit Kumar Pandey, Ashok Kumar, Dushyant Kumar Sharma, Ajay Kumar Yadav, Bablu Kumar, Abhishek, Vishal Chander, Gaurav Kumar Sharma, Ajay Pratap Singh and Chandan Prakash
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Subject |
Skin test (SICT) . Mycobacterium bovis . Tuberculosis . Buffaloes . ELISA . PCR
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Description |
Not Available
Bovine tuberculosis is an economically important disease with very high zoonotic potential. Single intradermal cervical tuberculin test (SICT) is considered a gold standard assay for the diagnosis of bovine tuberculosis. However, bovines especially buffaloes may produce a false negative result when the animal becomes cell-mediated immune (CMI) anergic in the advanced stage of the disease. In the present study, ELISA and PCR assays were successfully demonstrated to be useful in diagnosing tuberculosis especially in the CMI anergic buffaloes infected with Mycobacterium bovis. ELISA and PCR assays are able to detect 8.94% and 8.13%, respectively, more animals as positive in comparison to standard SICTassay in a selected population of 123 buffaloes. The moderate agreement between SICT and ELISA (k: 0.528; 0.249–0.807), a substantial agreement between SICT and PCR (k: 0.648; 0.364–0.931), and high agreement between ELISA and PCR (k: 0.856; 0.697–1.0) highlight that ELISA and PCR, if used in parallel with SICT, will provide better sensitivity over single assay. Reduction of false negative reactors may help in minimizing the zoonotic threat from bovine tuberculosis especially in disease endemic region where human and livestock interface is quite high. Not Available |
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Date |
2021-07-31T04:02:26Z
2021-07-31T04:02:26Z 2021-05-14 |
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Type |
Research Paper
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Identifier |
Joseph, B., Pandey, A.K., Kumar, A., Sharma, D.K., Yadav, A.K., Kumar, B., Chander, V., Sharma, G.K., Singh, A.P. and Prakash, C., 2021. Evaluation of ELISA and PCR in parallel to single intradermal cervical tuberculin test (SICT) for diagnosis of tuberculosis in buffaloes. Tropical Animal Health and Production, 53(2), pp.1-6.
https://doi.org/10.1007/s11250-021-02753-9 http://krishi.icar.gov.in/jspui/handle/123456789/51578 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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