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Evaluation of ELISA and PCR in parallel to single intradermal cervical tuberculin test (SICT) for diagnosis of tuberculosis in buffaloes

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Title Evaluation of ELISA and PCR in parallel to single intradermal cervical tuberculin test (SICT) for diagnosis of tuberculosis in buffaloes
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Creator Bincy Joseph, Amit Kumar Pandey, Ashok Kumar, Dushyant Kumar Sharma, Ajay Kumar Yadav, Bablu Kumar, Abhishek, Vishal Chander, Gaurav Kumar Sharma, Ajay Pratap Singh and Chandan Prakash
 
Subject Skin test (SICT) . Mycobacterium bovis . Tuberculosis . Buffaloes . ELISA . PCR
 
Description Not Available
Bovine tuberculosis is an economically important disease with very high zoonotic potential. Single intradermal cervical tuberculin test (SICT) is considered a gold standard assay for the diagnosis of bovine tuberculosis. However, bovines especially buffaloes may produce a false negative result when the animal becomes cell-mediated immune (CMI) anergic in the advanced stage of the disease. In the present study, ELISA and PCR assays were successfully demonstrated to be useful in diagnosing tuberculosis especially in the CMI anergic buffaloes infected with Mycobacterium bovis. ELISA and PCR assays are able to detect 8.94% and 8.13%, respectively, more animals as positive in comparison to standard SICTassay in a selected population of 123 buffaloes. The moderate agreement between SICT and ELISA (k: 0.528; 0.249–0.807), a substantial agreement between SICT and PCR (k: 0.648; 0.364–0.931), and high agreement between ELISA and PCR (k: 0.856; 0.697–1.0) highlight that ELISA and PCR, if used in parallel with SICT, will provide better sensitivity over single assay. Reduction of false negative reactors may help in minimizing the zoonotic threat from bovine tuberculosis especially in disease endemic region where human and livestock interface is quite high.
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Date 2021-07-31T04:02:26Z
2021-07-31T04:02:26Z
2021-05-14
 
Type Research Paper
 
Identifier Joseph, B., Pandey, A.K., Kumar, A., Sharma, D.K., Yadav, A.K., Kumar, B., Chander, V., Sharma, G.K., Singh, A.P. and Prakash, C., 2021. Evaluation of ELISA and PCR in parallel to single intradermal cervical tuberculin test (SICT) for diagnosis of tuberculosis in buffaloes. Tropical Animal Health and Production, 53(2), pp.1-6.
https://doi.org/10.1007/s11250-021-02753-9
http://krishi.icar.gov.in/jspui/handle/123456789/51578
 
Language English
 
Relation Not Available;
 
Publisher Springer