Record Details

Construction and characterization of yeast two-hybrid cDNA library derived from LFBK cell line

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Construction and characterization of yeast two-hybrid cDNA library derived from LFBK cell line
Not Available
 
Creator Sonalika Mahajan
Gaurav Kumar Sharma
Rakesh Matura
Saravanan Subramaniam
Jajati Keshari Mohapatra
Bramhadev Pattnaik
 
Subject FMD, SMART, Yeast two-hybrid, cDNA library
 
Description Not Available
The cDNA libraries are indispensable and critical tools for performing protein-protein interaction studies. In this study, a high quality yeast two-hybrid cDNA library from the LFBK cell line was constructed and characterized. LFBK cell line was originally derived from the swine kidney cells and is highly susceptible to foot-and-mouth disease virus (FMDV) infection. The total RNA was extracted from the LFBK cells and the switching mechanism at the 5' end of RNA template (SMART) technique was employed for the cDNA synthesis. Subsequently, double stranded cDNA was amplified by long-distance PCR, purified and co-transformed with pGADT7-rec vector in yeast strain Y187. The quality parameters of the constructed library were evaluated to qualify the constructed library. Nucleotide sequencing of the randomly selected clones from the library confirmed the swine genotype of LFBK cell line. The LFBK cDNA library was mated with the 2C protein of FMDV in yeast two-hybrid (YTH) system and several putative interaction partners were identified in the preliminary screening. The LFBK library was observed to be of high quality and could potentially be applied to protein interaction studies between FMDV and the host cells using YTH system.
Not Available
 
Date 2021-08-01T12:00:12Z
2021-08-01T12:00:12Z
2015-05
 
Type Research Paper
 
Identifier Mahajan S, Sharma GK, Matura R, Subramaniam S, Mohapatra JK, Pattnaik B. Construction and characterization of yeast two-hybrid cDNA library derived from LFBK cell line. Biologicals. 2015 May;43(3):202-8. doi: 10.1016/j.biologicals.2015.01.003. Epub 2015 Mar 16. PMID: 25787111.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/51828
 
Language English
 
Relation Not Available;
 
Publisher Elsevier