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Genetic Variability among sheep breeds by Random Amplified Polymorphic DNA-PCR.

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Title Genetic Variability among sheep breeds by Random Amplified Polymorphic DNA-PCR.
Not Available
 
Creator Kumar S.
Kolte AP
Yadav BR
Kumar S
Arora AL
Singh VK.
 
Subject Animal Nutrition
 
Description Not Available
Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was employed to assess the genetic variability and phylogenetic relationship among six breeds of sheep, viz., Malpura, Kheri, Chokla, Garole and two crossbreds Avikalin and Bharat Merino. Twenty-four individuals from each breed/crossbred were selected randomly. Initially, 40 primers were screened, of which 16 were found polymorphic and utilized for estimation of genetic variability and phylogenetic relationship among the breeds. The genetic distance was found highest between Malpura and Garole (D=0.1428), and the lowest (D=0.0612) between Avikalin and Chokla. However, the genetic identity was observed highest (I = 0.9406) between Avikalin and Chokla and the lowest (I = 0.8669) between Malpura and Garole. The Kheri sheep was found close with Chokla (D=0.0741), followed by Malpura sheep (D=0.0902) based on genetic distance. The phylogenetic tree also showed that Avikalin and Chokla are more close, whereas Malpura and Garole are distant to each other. The present study suggested that RAPD-PCR can be used successfully for analyzing genetic variation and phylogenetic relationship among breeds of sheep.
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Date 2021-08-03T08:23:34Z
2021-08-03T08:23:34Z
2008-10-01
 
Type Journal
 
Identifier Kumar S., Kolte AP., Yadav BR., Kumar S., Arora AL. and Singh VK. (2008). Genetic Variability among sheep breeds by Random Amplified Polymorphic DNA-PCR. Indian Journal of Biotechnology, 5: 482-485
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http://krishi.icar.gov.in/jspui/handle/123456789/52549
 
Language English
 
Relation Not Available;
 
Publisher CSIR