Effect of vitrification medium composition and exposure time on post thaw development of in vitro produced buffalo embryos.
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Title |
Effect of vitrification medium composition and exposure time on post thaw development of in vitro produced buffalo embryos.
Not Available |
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Creator |
Manjunatha BM
Gupta PSP Ravindra JP Devaraj M Nandi S |
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Subject |
In vitro production
Buffalo EmbryoVitrification |
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Description |
Not Available
This study examined the effects of different vitrification medium compositions and exposure times (2, 4 and 6min) on the post-thaw development of buffalo embryos produced in vitro (IVP). The compositions were (1) 40% ethylene glycol (EG); (2) 25% glycerol (G)+25% EG, and (3) 25% EG+25% dimethylsulfoxide (DMSO). The base medium was 25mM Hepes-buffered TCM-199+10% steer serum +50microg/mL gentamycin. The IVP embryos were cryopreserved by a two-step vitrification method at 24 degrees C. After warming, the embryos were cultured in vitro for 72h. The vitrification of morulae and blastocysts in 25% EG+25% DMSO with an exposure time of 2 and 4min, respectively, resulted in a better hatching rate than other combinations. The hatching rate of morulae vitrified in 25% EG+25% G, 25% EG+25% DMSO, and blastocysts vitrified in 40% EG, 25% EG+25% DMSO were negatively correlated with exposure time. However, the hatching rate of blastocysts vitrified in 25% EG+25% G was positively correlated with exposure time. The study demonstrated that the post-thaw in vitro development of IVP buffalo embryos was affected by the vitrification medium composition and exposure time. Not Available |
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Date |
2021-08-05T03:22:28Z
2021-08-05T03:22:28Z 2009-01-01 |
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Type |
Journal
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Identifier |
Manjunatha BM, Gupta PSP, Ravindra JP, Devaraj M and Nandi S (2009) Effect of vitrification medium composition and exposure time on post thaw development of in vitro produced buffalo embryos. The Veterinary Journal. 179:287-291.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/53132 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier Ltd
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