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Effect of vitrification medium composition and exposure time on post thaw development of in vitro produced buffalo embryos.

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Title Effect of vitrification medium composition and exposure time on post thaw development of in vitro produced buffalo embryos.
Not Available
 
Creator Manjunatha BM
Gupta PSP
Ravindra JP
Devaraj M
Nandi S
 
Subject In vitro production
Buffalo
EmbryoVitrification
 
Description Not Available
This study examined the effects of different vitrification medium compositions and exposure times (2, 4 and 6min) on the post-thaw development of buffalo embryos produced in vitro (IVP). The compositions were (1) 40% ethylene glycol (EG); (2) 25% glycerol (G)+25% EG, and (3) 25% EG+25% dimethylsulfoxide (DMSO). The base medium was 25mM Hepes-buffered TCM-199+10% steer serum +50microg/mL gentamycin. The IVP embryos were cryopreserved by a two-step vitrification method at 24 degrees C. After warming, the embryos were cultured in vitro for 72h. The vitrification of morulae and blastocysts in 25% EG+25% DMSO with an exposure time of 2 and 4min, respectively, resulted in a better hatching rate than other combinations. The hatching rate of morulae vitrified in 25% EG+25% G, 25% EG+25% DMSO, and blastocysts vitrified in 40% EG, 25% EG+25% DMSO were negatively correlated with exposure time. However, the hatching rate of blastocysts vitrified in 25% EG+25% G was positively correlated with exposure time. The study demonstrated that the post-thaw in vitro development of IVP buffalo embryos was affected by the vitrification medium composition and exposure time.
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Date 2021-08-05T03:22:28Z
2021-08-05T03:22:28Z
2009-01-01
 
Type Journal
 
Identifier Manjunatha BM, Gupta PSP, Ravindra JP, Devaraj M and Nandi S (2009) Effect of vitrification medium composition and exposure time on post thaw development of in vitro produced buffalo embryos. The Veterinary Journal. 179:287-291.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/53132
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Ltd