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Isolation and culture of ovine and bubaline small and large preantral follicles: effect of cyclicity and presence of a dominant follicle.

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Title Isolation and culture of ovine and bubaline small and large preantral follicles: effect of cyclicity and presence of a dominant follicle.
Not Available
 
Creator Nandi S
Girish Kumar V
Manjunatha BM
Ramesh HS
Gupta PSP
 
Subject ovine
preantral follicles
bubaline
 
Description Not Available
Studies were conducted to examine the effects of the cyclicity and the presence of a dominant follicle (DF) in ovary on the recovery and in vitro growth of pre-antral follicles (PFs) in sheep and buffalo. Small pre-antral follicles (SPFs, 100– 250 lm) and large pre-antral follicles (LPFs, 250–450 lm) were isolated from slaughterhouse ovaries in the breeding seasons by a mechanical and enzymatic method. The sheep and buffalo PFs were cultured in vitro for 6 and 15 days, respectively, and examined for their growth, survival and antrum formation rates and growth rates of oocytes in cultured preantral follicles. The follicles of the sheep and buffalo were
recovered and cultured simultaneously within replicates. The recovery rates (number per ovary) of both SPFs and LPFs were significantly (p < 0.05) higher in cyclic ewes (SPFs: 22.0 ± 3.3 vs 12.1 ± 2.6 and LPFs: 16.0 ± 3.6 vs 9.2 ± 1.8) and buffaloes (SPFs: 9.2 ± 1.3 vs 4.1 ± 1.0 and LPFs: 10.3 ± 2.7 vs 5.4 ± 0.7) compared with those recovered from acyclic ones. Presence of a DF in ovary significantly (p < 0.05) reduced the recovery rates of LPFs in ewes (9.06 ± 2.7 vs 16.4 ± 3.8) but had no effect in buffalo. Cyclicity of animals or follicular dominance had no effects on in vitro growth, survival and antrum formation rates and growth rates of oocytes in
cultured PFs of SPFs and LPFs in both sheep and buffalo. The in vitro growth, survival and antrum formation rates of LPFs and growth rates of oocytes in cultured LPFs were significantly (p < 0.05) higher than those observed in SPFs in both sheep and buffalo. The overall recovery and growth rates of the PFs were lower in buffaloes compared with ewes
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Date 2021-08-05T08:48:24Z
2021-08-05T08:48:24Z
2009-01-01
 
Type Journal
 
Identifier Nandi S, Girish Kumar V, Manjunatha BM, Ramesh HS and Gupta PSP (2009) Isolation and culture of ovine and bubaline small and large preantral follicles: effect of cyclicity and presence of a dominant follicle. Reproduction in Domestic Animals 44:74-79.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/53275
 
Language English
 
Relation Not Available;
 
Publisher John Wiley & Sons, Inc