Chlorpyrifos and endosulfan affect buffalo oocyte maturation, fertilization, and embryo development in vitro directly and through cumulus cells
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Chlorpyrifos and endosulfan affect buffalo oocyte maturation, fertilization, and embryo development in vitro directly and through cumulus cells
|
|
Creator |
Nandi S
Gupta PSP Roy SC Selvaraju S Ravindra JP |
|
Subject |
Animal physiology
|
|
Description |
Not Available
This study was undertaken to examine the effect of 10 different levels (0, 0.005, 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, 2.0, and 4.0 μg/mL) of two pesticides (chlorpyrifos and endosulfan) on buffalo oocyte viability, maturation, fertilization, and developmental competences in vitro. Studies were conducted to test the development of oocytes cultured with pesticides during maturation, fertilization, and during different embryo development stages. We also conducted experiments to test the hypotheses that the effects of these pesticides are hormones and somatic cells mediated. We observed a dose dependant decline in viability and developmental competence rates of oocytes. Chlorpyrifos and endosulfan had a negative impact on oocytes at 0.02 and 0.1 μg/mL levels, respectively. These pesticides reduced the oocyte nuclear maturation by a direct effect on oocytes, cumulus cell-mediated action, and by blocking the action of hormones. Chlorpyrifos was found to be more ovotoxic and embryotoxic than endosulfan. This study will provide information on dose-response relationship and risk assessment in domestic buffaloes. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 57–67, 2011. Not Available |
|
Date |
2021-08-11T03:45:33Z
2021-08-11T03:45:33Z 2011-02-10 |
|
Type |
Journal
|
|
Identifier |
Nandi S, Gupta PSP, Roy SC, Selvaraju S and Ravindra JP. 2011. Chlorpyrifos and endosulfan affect buffalo oocyte maturation, fertilization, and embryo development in vitro directly and through cumulus cells. Environmental Toxicology, 26:57-67.
http://krishi.icar.gov.in/jspui/handle/123456789/54598 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
John Wiley & Sons, Inc
|
|