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Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labeling technique

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Title Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labeling technique
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Creator Sreejalekshmi P
Raghavendra BS
Siva Subramani T
Murthy VC
Jamuna KV
Prasad RV
Ravindra JP
Selvaraju S
 
Subject Animal physiology
 
Description Not Available
The objective of this experiment was to assess the features and extent of follicular apoptosis in the water buffalo (Bubalus bubalis) ovary using classical histology and nick end labelling technique. Ovaries (n = 40) procured from the slaughterhouse were used for the study. The sections (5 μm) were used for detection of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) and classical histology (H&E). Those follicles showing ≥ 5% TUNEL positivity (TUNEL assay) and pyknotic nuclei (histology) in granulosa cells were classified as atretic. Based on histology, the atretic primary and secondary follicles (%) were 93.82 and 95.62 respectively. The histology study reveals that the rates (%) of atresia in 5 mm were 36.90, 40.50, 62.84 and 74.5 respectively. Further the atretic tertiary follicles (%) were significantly lower than the primary and secondary classes of follicles. TUNEL assay reveals that the atretic rate (%) of tertiary follicles in 5 mm diameter follicles were significantly lower in TUNEL than histology. Percentages of granulosa and thecal cells positive for atresia by TUNEL were 30.7 ± 0.53 and 13.82 ± 0.18 respectively per follicle. The initial structural changes in atretic follicles were seen primarily in the granulosa cells. In severely atretic follicles TUNEL positive granulosa cells along with theca cells have to be considered in assessing the rate and extent of atresia.
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Date 2021-08-10T09:22:32Z
2021-08-10T09:22:32Z
2011-01-18
 
Type Journal
 
Identifier Sreejalekshmi P, Raghavendra BS, Siva Subramani T, Murthy VC, Jamuna KV, Prasad RV, Ravindra JP and Selvaraju S. 2011. Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labeling technique. Reproduction in Domestic Animals, 46:59- 65.. 2011. Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labeling technique. Reproduction in Domestic Animals, 46:59- 65.
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http://krishi.icar.gov.in/jspui/handle/123456789/54225
 
Language English
 
Relation Not Available;
 
Publisher John Wiley & Sons, Inc