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Characterizing miRNA and mse-ts RNA in fertile and sub-fertile yak bull spermatozoa from Arunachal Pradesh

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Title Characterizing miRNA and mse-ts RNA in fertile and sub-fertile yak bull spermatozoa from Arunachal Pradesh
Not Available
 
Creator Das Parth Partim
Begum S. S.
Choudhury Manasee
Medhi D.
Paul Vijay
Das P. J.
 
Subject yak sperm
miRNA142
miRNA143
 
Description Not Available
Male fertility in farm animals is considered as an important economic trait. The phenomenon of spermatogenesis plays a dynamic functional role in determining the viability of sperm and thereby can impact on fertility-driven complications. The process of spermatogenesis is controlled by numerous molecular factors and requires a precisely regulated pattern of gene expression. The role of small noncoding RNAs in altering gene expression has been extensively studied. However, limited information is available apropos their role in yak spermatogenesis. The present study aimed to evaluate the assessment of some significant microRNAs and their expression pattern in the body tissues and sperm of fertile and subfertile yak from Arunachal Pradesh besides identified a novel class of sperm enriched small RNA ‘mature-sperm-enriched small RNA’ (mse-tsRNA) in Yak spermatozoa. The RNA was extracted from tissue and sperm using 27 gauge needles and subsequently reverse transcribed into small RNA cDNAs. The PCR positive sperm-predominant miRNAs were validated by quantitative reverse transcriptase PCR (qRT-PCR) for their expression in fertile and subfertile yak. Of the 22 microRNAs, the miRNA19a, miRNA142 and miRNA143 showed higher expression in the subfertile yak, whereas expression of miRNA7d, miRNA23a and miRNA23b were found elevated in the fertile animal. The presence of these small noncoding RNAs in yak sperm and testis indicated the legitimate involvement of their role in yak bull fertility.
Not Available
 
Date 2021-08-10T11:09:37Z
2021-08-10T11:09:37Z
2021-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/54286
 
Language English
 
Relation Not Available;
 
Publisher Not Available