ICAR Research Data Repository for Knowledge Management
Distinction between glycomacropeptide and β-lactoglobulin with ‘stains all’ dye on Tricine SDS-PAGE gels.
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View Archive Info| Field | Value | |
| Title |
Distinction between glycomacropeptide and β-lactoglobulin with ‘stains all’ dye on Tricine SDS-PAGE gels.
Not Available |
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| Creator |
Sharma, N.; Sharma, R.; Rajput, Y.S.; Mann, B. and Gandhi, K.
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| Subject |
Glycomacropeptide, β-Lactoglobulin‘Stains all’ dye, SDS-PAGE, Whey
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| Description |
Not Available
Identification of glycomacropeptide (GMP) and β-lactoglobulin (β-lg) present in cheese whey is difficult on SDS-PAGE due to their close proximity during electrophoresis and poor sensitivity of commonly used staining dye ‘coomassie brilliant blue’ (CBB) towards GMP. A simple method has been developed for the detection of GMP and β-lg by staining acrylamide gel after tricine SDS-PAGE using cationic ‘stains all’ dye. After staining and destaining major whey proteins, viz. ɑ-lactalbumin (ɑ-la) and β-lg appear red while GMP stains blue. The method can be used for the identification of these macromolecules in cheese whey and the detection of adulteration of milk with rennet whey. Not Available |
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| Date |
2021-08-26T08:36:36Z
2021-08-26T08:36:36Z 2021-03-15 |
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| Type |
Research Paper
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60785 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Elsevier
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