Record Details

Separation methods for milk proteins on polyacrylamide gel electrophoresis: Critical analysis and options for better resolution.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Separation methods for milk proteins on polyacrylamide gel electrophoresis: Critical analysis and options for better resolution.
Not Available
 
Creator Sharma, N.; Sharma, R.; Rajput, Y.S.; Mann, B.; Singh, R. and Gandhi, K.
 
Subject polyacrylamide gel electrophoresis
 
Description Not Available
Over the years, a number of methodological variations in polyacrylamide gel electrophoresis (PAGE) have
been attempted for the separation of milk proteins. Caseins are similar in size and differ very little in net
charge from each other. Also, in heated milk, k-casein not only complexes with whey proteins but also
undergoes self-polymerisation. These cause problems in the resolution of milk proteins and encouraged
researchers to modify PAGE. Seven different types of PAGE, i.e., native-PAGE, urea-PAGE, reducing sodium dodecyl sulphate (SDS)-PAGE, non-reducing-SDS-PAGE, urea-SDS-PAGE, tricine-PAGE, and two dimensional electrophoresis (2DE), are reviewed with particular emphasis on the pattern of milk pro tein electrophoretograms, merits, demerits and applications. Although the literature suggests that SDS PAGE is increasingly used for separating milk proteins, other forms of PAGE have their own advantage.
This review can act as a quick screening aid for researchers in selecting an appropriate type of PAGE for
the separation of milk proteins for different purposes.
Not Available
 
Date 2021-08-26T08:36:46Z
2021-08-26T08:36:46Z
2021-03-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/60786
 
Language English
 
Relation Not Available;
 
Publisher Elsevier