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Applicability of protein estimation methods for assaying glycomacropeptide

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Title Applicability of protein estimation methods for assaying glycomacropeptide
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Creator Narwal, V.; Sharma, N.; Sharma, R.; Rajput, Y.S.; Mann, B.
 
Subject Glycomacropeptide, milk, protein
 
Description Not Available
Glycomacropeptide (GMP) is a nutraceutical additive, which is released during cheese preparation. In this study, the protein content in a laboratory GMP preparation and two commercial GMP samples, which was estimated by the Bradford, biuret, Lowry and bicinchoninic acid (BCA) assays, were compared using the Kjeldahl method. There were apparent differences in the values of estimated proteins using the different methods. An absence of dose response in the Bradford method and a low dose response using the Lowry method indicated the unsuitability of both these methods. Absorbance in the biuret and BCA methods linearly increased with increasing GMP content. The BCA method was the most sensitive of these methods.
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Date 2021-08-26T08:32:58Z
2021-08-26T08:32:58Z
2017-09-15
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/60764
 
Language English
 
Relation Not Available;
 
Publisher Wiley Online Libraryr