Applicability of protein estimation methods for assaying glycomacropeptide
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Title |
Applicability of protein estimation methods for assaying glycomacropeptide
Not Available |
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Creator |
Narwal, V.; Sharma, N.; Sharma, R.; Rajput, Y.S.; Mann, B.
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Subject |
Glycomacropeptide, milk, protein
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Description |
Not Available
Glycomacropeptide (GMP) is a nutraceutical additive, which is released during cheese preparation. In this study, the protein content in a laboratory GMP preparation and two commercial GMP samples, which was estimated by the Bradford, biuret, Lowry and bicinchoninic acid (BCA) assays, were compared using the Kjeldahl method. There were apparent differences in the values of estimated proteins using the different methods. An absence of dose response in the Bradford method and a low dose response using the Lowry method indicated the unsuitability of both these methods. Absorbance in the biuret and BCA methods linearly increased with increasing GMP content. The BCA method was the most sensitive of these methods. Not Available |
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Date |
2021-08-26T08:32:58Z
2021-08-26T08:32:58Z 2017-09-15 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60764 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Wiley Online Libraryr
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