Record Details


Field	Value

Title	A method for estimation of urea using ammonia electrode and its applicability to milk samples. Not Available

Creator	Sharma, R.; Rajput, Y.S.; Kaur, S. and Tomar, S.K.

Subject	urea, milk

Description	Not Available A method for the estimation of urea in milk using ammonia electrode is described. Urea is first degraded by urease enzyme into ammonium ion and carbon dioxide at neutral pH. The ammonium ion is then converted into ammonia at alkaline pH. A linear inverse relationship was observed between logarithmic concentration of ammonia or urea and electrode response. Repeatability, expressed as a coefficient of variation, was 1.77% at a level of 8.92 mm-urea in milk. The method was validated in milk samples spiked with between 2 x 10-3 and 10 x 10-3 m-urea and recovery of added urea was quantitative. Whereas, preservative sodium azide at 0.5 g/l or 2 g/l level did not affect results, lower values of urea concentration in presence of Bronopol at 0.5 g/l were observed. Urea levels in milk samples estimated by this method were comparable to standard enzymatic method. The method is simple, fast and is not prone to interference from other milk constituents. Not Available

Date	2021-08-26T08:26:34Z 2021-08-26T08:26:34Z 2008-11-01

Type	Research Paper

Identifier	Not Available Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60719

Language	English

Relation	Not Available;

Publisher	Not Available

ICAR Research Data Repository for Knowledge Management