Deciphering the complexity of sperm transcriptome reveals genes governing functional membrane and acrosome integrities potentially influence fertility
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Title |
Deciphering the complexity of sperm transcriptome reveals genes governing functional membrane and acrosome integrities potentially influence fertility
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Creator |
S.Selvaraju
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Subject |
sperm fertility,Transcriptome analysis,semen quality
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Description |
Not Available
Deciphering sperm transcriptome is the key to understanding the molecular mechanisms governing peri-fertilization, embryonic development, and pregnancy establishment. This study aimed to profile sperm transcriptome to identify signature transcripts regulating male fertility. Semen samples were collected from 47 bulls with varied fertility rates. The sperm total RNA was isolated (n?=?8) and subjected to transcriptome sequencing. Based on the expression pattern obtained from RNA profiling, the bulls were grouped (p?=?0.03) into high-fertile and sub-fertile, and signature transcripts controlling sperm functions and fertility were identified. The results were validated using the OMIM database, qPCR, and sperm function tests. The sperm contains 1100 to 1700 intact transcripts, of which BCL2L11 and CAPZA3 were abundant and associated (p? |
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Date |
2021-08-25T11:04:42Z
2021-08-25T11:04:42Z 2021-1-1 |
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Type |
Research Paper
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Identifier |
Selvaraju, S., Ramya, L., Parthipan, S. et al. Deciphering the complexity of sperm transcriptome reveals genes governing functional membrane and acrosome integrities potentially influence fertility. Cell Tissue Res 385, 207?222 (2021). https://doi.org/10.1007/s00441-021-03443-6
Not Available 0302-766X http://krishi.icar.gov.in/jspui/handle/123456789/60535 |
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Language |
English
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Publisher |
Not Available
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