Regenerated silica-based RNA purification columns to address the short supply of RNA purification kits for COVID-19 diagnosis
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Title |
Regenerated silica-based RNA purification columns to address the short supply of RNA purification kits for COVID-19 diagnosis
Not Available |
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Creator |
Jitendra Kumar Biswal
Rajeev Ranjan Shyam Singh Dahiya Smrutirekha Mallick Jajati K. Mohapatra |
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Subject |
SARS-CoV2 · COVID-19 · RT-qPCR · RNA-extraction · Regenerated RNA-purification column
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Description |
Not Available
Background RT-qPCR technique is the current world-wide method used for the early detection of SARS-CoV2 RNA in the suspected clinical samples. Viral RNA extraction is the key pre-analytical step for SARS-CoV2 detection which often achieved using commercial RNA-extraction kits. However, due to the COVID-19 pandemic, bulk production and the supply chains for the commercial RNA-extraction kit have been seriously compromised. The shortage of commercial RNA-extraction kit is even more acute in developing country. Furthermore, use of one-off design RNA-columns can generate plastic wastes that have an environmental pollution effect. Methods and results To address these issues, in this study, we used warm alkaline solution containing Triton X-100 for the complete removal of the residual SARS-CoV2 RNA from the used RNA-binding silica column. Columns regenerated using the alkaline solution have the viral RNA purification capability that is comparable to the fresh silica columns. We also demonstrated that RNA-binding silica columns can be regenerated and reused for a minimum of five-times. Conclusions Therefore, the use of the RNA-column regeneration method may benefits several SARS-CoV2 diagnostic laboratories throughout the world by cutting down the requirement of commercial RNA-purification column. Not Available |
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Date |
2022-01-14T08:29:11Z
2022-01-14T08:29:11Z 2021-09-12 |
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Type |
Research Paper
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Identifier |
Biswal, J.K., Ranjan, R., Dahiya, S.S. et al. Regenerated silica-based RNA purification columns to address the short supply of RNA purification kits for COVID-19 diagnosis. Mol Biol Rep 48, 6871–6877 (2021). https://doi.org/10.1007/s11033-021-06688-0
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/68669 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer Nature
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