Record Details

Identification of immuno-dominant antigens of Trypanosoma evansi for detection of chronic trypanosomosis using experimentally infected equines

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Identification of immuno-dominant antigens of Trypanosoma evansi for detection of chronic trypanosomosis using experimentally infected equines
Not Available
 
Creator Not Available
S.C. Yadav, R. Kumar, Vipin Kumar, Jaideep, Ritesh Kumar, A.K. Gupta, B.C. Bera, U. Tatu
 
Subject Trypanosoma evansi; Proteome; Immunodominant; Trypanosomosis; ELISA; Immunoblot
 
Description Not Available
Trypanosoma evansi is the most extensively distributed trypanosome responsible for disease called surra
in livestock in many countries including frequent outbreaks in India. The prevalence of this disease is
most commonly reported by standard parasitological detection methods (SPDM); however, antibody
ELISA is being in practice by locally produced whole cell lysate (WCL) antigens in many countries. In
the present investigation, we attempted to identify and purify immuno dominant, infection specific trypanosome
antigens from T. evansi proteome using experimentally infected equine serum by immuno
blot. Three immuno dominant clusters of proteins i.e. 62–66 kDa, 52–55 kDa and 41–43 kDa were identified
based on their consistent reactivity with donkey sequential serum experimentally infected T. evansi
up to 280 days post infection (dpi). The protein cluster of 62–66 kDa was purified in bulk in native form
and comparatively evaluated with whole cell lysate antigen (WCL). ELISA and immuno blot showed that
polypeptide of this cluster is 100% sensitive in detection of early and chronic infection. Further, this protein
cluster was also found immuno reactive against hyper immune serum raised against predominantly
66 kDa exo antigen, revealed that this is a common immunodominant moieties in proteome and secretome
of T. evansi.
Not Available
 
Date 2022-09-29T09:48:25Z
2022-09-29T09:48:25Z
2013-05-25
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/74616
 
Language English
 
Relation Not Available;
 
Publisher Elsevier