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Cytotoxicity and genotoxicity of a trypanocidal drug quinapyraminesulfate loaded-sodium alginate nanoparticles in mammalian cells

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Title Cytotoxicity and genotoxicity of a trypanocidal drug quinapyraminesulfate loaded-sodium alginate nanoparticles in mammalian cells
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Creator Anju Manuja, Balvinder Kumar, Meenu Chopra, Anshu Bajaj, Rajender Kumara, Neeraj Dilbaghi, Sandeep Kumar, Sandeep Singh, T. Riyesh, Suresh C. Yadav
 
Subject Trypanosoma; Nanomedicine; Genotoxicity; Cytotoxicity; Oxidative stress
 
Description Not Available
We synthesized quinapyramine sulfate loaded-sodium alginate nanoparticles (QS-NPs) to reduce unde-sirable toxic effects of QS against the parasite Trypanosoma evansi, a causative agent of trypanosomosis. To determine the safety of the formulated nanoparticles, biocompatibility of QS-NPs was determined using Vero, Hela cell lines and horse erythrocytes in a dose-dependent manner. Our experiments unveiled a concentration-dependent safety/cytotoxicity (metabolic activity), genotoxicity (DNA damage, chromosomal aberrations), production of reactive oxygen species and hemolysis in QS-NPs treated cells. Annexin-Vpropidium iodide (PI) staining showed no massive apoptosis or necrosis. However, at very high doses(more than 300 times than the effective doses), we observed more toxicity in QS-NPs treated cells as compared to QS treated cells. QS-NPs were safe at effective trypanocidal doses and even at doses several times higher than the effective dose.
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Date 2022-09-29T09:49:29Z
2022-09-29T09:49:29Z
2016-03-18
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/74620
 
Language English
 
Relation Not Available;
 
Publisher Elsevier