Record Details

Microsatellite genotyping of Trypanosoma evansi isolates from livestock of different agro-ecological zones of India

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Microsatellite genotyping of Trypanosoma evansi isolates from livestock of different agro-ecological zones of India
Not Available
 
Creator VIJESH KUMAR SAINI, JARNAIL SINGH, JYOTI KAUSHIK, SOUTI PRASAD SARKHEL, SANJAY KUMAR, S C YADAV, A K GUPTA and RAJENDER KUMAR
 
Subject Genotyping, India, Livestock, Microsatellite loci, Polymorphism, Surra, Trypanosoma evansi
 
Description Not Available
The present study deals with genetic diversity analysis by microsatellite genotyping of Trypanosoma evansi
isolates collected from different livestock hosts (horse, donkey, camel and cattle) inhabiting different geographical
location in North and North-western parts of India. Out of 12 microsatellite loci used in the study 10 successfully
genotyped 6 T. evansi isolates, and 6 of them were found polymorphic. At 6 microsatellite loci (TB8/11, TB11/29,
TB10/1, TB1/8, TB3/3 and TB4/2) observed number of alleles per locus ranged from 3 (TB8/11) to 6 (TB4/2) with
a mean of 4.83±1.75 and effective number of alleles ranged from 2.11 (TB8/1) to 5.00 (TB4/2) with a mean value
of 3.32±1.16. At 4 microsatellite loci (TB11/13, TB2/19, TB11/1 and TB8/1) observed (na=2) as well as effective
(ne=2) number of alleles were the same. The observed heterozygosity varied from 0.16 (TB8/11) to 1.00 (TB11/13,
TB2/19, TB10/1, TB1/8 and TB4/2) with a mean of 0.76±0.37. The expected value of heterozygosity (Levene’s)
varied from 0.48 (TB8/1) to 0.88 (TB4/2) with a mean of 0.59±0.23. Polymorphism information content (PIC)
value ranged from 0.34 (TB8/1) to 0.77 (TB4/2), indicating that TB11/29, TB10/1, TB1/8, TB3/3 and TB4/2
microsatellite loci were highly informative (PIC>0.5). Neighbour-joining tree analysis indicated different clustering
of isolates based on allelic sharing. In the present study, 6 microsatellite markers were found useful to determine
genetic diversity among Indian T. evansi isolates.
Not Available
 
Date 2022-09-29T09:53:14Z
2022-09-29T09:53:14Z
2014-07-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/74643
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research