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Genetic differentiation amongst different Trypanosoma evansi isolates using random primer DNA typing

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Title Genetic differentiation amongst different Trypanosoma evansi isolates using random primer DNA typing
Not Available
 
Creator RAJENDER KUMAR, PARVATI SHARMA, DEEPAK KUMAR GAUR, SACHIN KUMAR GOYAL and SANJAY KUMAR
 
Subject DNA, Genetic variability, RAPD, Surra, Trypanosoma evansi
 
Description Not Available
Trypanosomosis (surra) caused by Trypanosoma evansi
is one of the important diseases of livestock including
equines. The disease has the widest geographical range of
all the pathogenic trypanosome species and is a major
constraint to livestock productivity on the three continents,
Asia, Africa and South America. T. evansi infects a wide
range of domestic and wild animals in India, viz. camels,
horses, donkeys, mules, cattle, buffaloes, dogs, pigs, goats,
tigers and elephants. It is hypothesized that T. evansi has
evolved from T. brucei when infected camels moved to tse
tse free areas and adapted to survive without natural
biological vector and transformed to mechanical
transmission through haematophagus biting flies and thus
existence of sexual recombination in this species is highly
unlikely and follow clonal propagation population structure.
The variations in host susceptibility, virulence of different
isolates are commonly observed conditions in animals
suffering from surra. Therefore, study of genetic variability
among different T. evansi isolates is very important to know
molecular epidemiology, and formulation of prevention and
control strategies of the disease. For specific detection and
molecular typing of blood protozoan, random amplification
of polymorphic DNA (RAPD) technique has gained
importance in recent years. The present study was carried
out to know genetic variability among Indian T. evansi
isolates of different hosts and agro-ecological regions using
RAPD- PCR approach with new and already reported
informative primers.
Not Available
 
Date 2022-09-29T09:53:27Z
2022-09-29T09:53:27Z
2015-11-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/74645
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research