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Recombinant non-structural polyprotein 3AB-based serodiagnostic strategy for FMD surveillance in bovines irrespective of vaccination

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Title Recombinant non-structural polyprotein 3AB-based serodiagnostic strategy for FMD surveillance in bovines irrespective of vaccination
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Creator Jajati K Mohapatra
Laxmi K Pandey
Aniket Sanyal
Bramhadev Pattnaik
 
Subject FMD serosurveillance, Recombinant 3AB, Indirect ELISA
 
Description Not Available
In India, the proportion of bovines vaccinated against foot-and-mouth disease (FMD) is increasing since the implementation of the Government supported ‘FMD Control Programme’, and non-structural protein (NSP)-based serological assays for discriminating between antibodies induced by infection or vaccination (DIVA) could be useful. The FMD virus NSP 3AB was expressed in a prokaryotic system and an indirect ELISA (r3AB3 I-ELISA) was developed and validated as a screening assay for detecting virus in vaccinated bovines. The diagnostic sensitivity of the assay was estimated to be 96%, while the diagnostic specificity varied between the naïve and vaccinates as 99.1% and 96.4%, respectively. This assay could detect antibodies to 3AB (3AB-Ab) from 10 to as late as 900 days post-infection in cattle infected experimentally. The “in-house” assay demonstrated higher sensitivity than a commercial 3ABC ELISA kit particularly with samples obtained from the late stages of infection. Transient post-vaccinal 3AB-Ab response could be detected in one of the three commercial vaccines during the six-month vaccination regimen, which emphasizes the fact that for a DIVA-compatible diagnostic strategy to be a realistic option, all vaccines need to be quality checked for the NSP content.
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Date 2021-08-05T04:43:36Z
2021-08-05T04:43:36Z
2011-11-01
 
Type Research Paper
 
Identifier Mohapatra JK, Pandey LK, Sanyal A, Pattnaik B. Recombinant non-structural polyprotein 3AB-based serodiagnostic strategy for FMD surveillance in bovines irrespective of vaccination. J Virol Methods. 2011 Nov;177(2):184-92. doi: 10.1016/j.jviromet.2011.08.006. Epub 2011 Aug 11. PMID: 21864578.
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http://krishi.icar.gov.in/jspui/handle/123456789/53142
 
Language English
 
Relation Not Available;
 
Publisher Elsevier