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Multiplex PCR for rapid detection of serotype A foot-and-mouth disease virus variants with amino acid deletion at position 59 of the capsid protein VP3

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Title Multiplex PCR for rapid detection of serotype A foot-and-mouth disease virus variants with amino acid deletion at position 59 of the capsid protein VP3
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Creator Jajati K Mohapatra
Laxmi K Pandey
Gaurav K Sharma
Sushanta K Barik
Sachin S Pawar
Rameshkumar Palsamy
Bramhadev Pattnaik
 
Subject FMD virus type A, VP359-deletion group, Rapid detection, Multiplex PCR
 
Description Not Available
In India, there has been co-circulation, extinction and emergence of genotypes/lineages within serotype A foot-and-mouth disease (FMD) virus. At present an antigenically heterogeneous, unique lineage within genotype VII dominates the field outbreaks. This genetic cluster has amino acid deletion at position 59 of VP3 (VP359-deletion group), considered to be critical antigenically. The emergence of this group warrants rapid and accurate detection to facilitate early planning and implementation of an effective control policy. A rapid multiplex PCR assay was developed for detection of the dominating VP359-deletion group with 100% sensitivity and specificity, even before generating sequence data and confirmatory phylogenetic analysis. This development is important for surveillance of FMD in India.
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Date 2021-08-05T04:51:29Z
2021-08-05T04:51:29Z
2011-01-01
 
Type Research Paper
 
Identifier Mohapatra JK, Pandey LK, Sharma GK, Barik SK, Pawar SS, Palsamy R, Pattnaik B. Multiplex PCR for rapid detection of serotype A foot-and-mouth disease virus variants with amino acid deletion at position 59 of the capsid protein VP3. J Virol Methods. 2011 Jan;171(1):287-91. doi: 10.1016/j.jviromet.2010.10.016. Epub 2010 Oct 26. PMID: 21029752.
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http://krishi.icar.gov.in/jspui/handle/123456789/53146
 
Language English
 
Relation Not Available;
 
Publisher Elsevier