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Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease

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Title Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease
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Creator Jajati Keshari Mohapatra
Saravanan Subramaniam
Chakradhar Tosh
Divakar Hemadri
Aniket Sanyal
Tamilselvan Ramasamy Periyasamy
Thaha Jamal Rasool
 
Subject FMD virusSerotype AGenotype differentiationRT-PCRSandwich ELISA
 
Description Not Available
Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country.
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Date 2021-08-12T04:49:28Z
2021-08-12T04:49:28Z
2007-07
 
Type Research Paper
 
Identifier Mohapatra JK, Subramaniam S, Tosh C, Hemadri D, Sanyal A, Periyasamy TR, Rasool TJ. Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease. J Virol Methods. 2007 Jul;143(1):117-21. doi: 10.1016/j.jviromet.2007.02.008. Epub 2007 Apr 2. PMID: 17400298.
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http://krishi.icar.gov.in/jspui/handle/123456789/55156
 
Language English
 
Relation Not Available;
 
Publisher Elsevier