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Truncated recombinant non-structural protein 2C-based indirect ELISA for FMD sero-surveillance

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Title Truncated recombinant non-structural protein 2C-based indirect ELISA for FMD sero-surveillance
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Creator Sonalika Mahajan
Jajati Keshari Mohapatra
Laxmi Kant Pandey
Gaurav Kumar Sharma
Bramhadev Pattnaik
 
Subject 2C, DIVA, Foot-and-mouth disease, NSP
 
Description Not Available
Foot-and-mouth disease (FMD) is a transboundary animal disease caused by foot-and-mouth disease virus. In India, systematic preventive vaccination using inactivated trivalent (O, A and Asia 1) vaccine is the strategy being adopted to control FMD. The use of non-structural protein (NSP)-contaminated inactivated vaccine raises concerns over differentiation of infected and vaccinated animals (DIVA) by NSP based immunoassays. However, 2C being a membrane associated protein usually remain absent in vaccine formulations and thus, anti-2C response is one of the most reliable indicator of the FMDV infection. In this study, 34 amino acids from N-terminus of 2C protein were removed to eliminate membrane-binding amphipathic helicase activity for the expression of recombinant protein in soluble form. Truncated 2C (2Ct) was utilized for development of an indirect ELISA (I-ELISA) for bovine and the developed 2Ct I-ELISA was validated using a panel constituting of serum of naïve, vaccinated and infected animals. The assay was compared with the in-house r3AB3 I-ELISA and the overall concordance was 85.31%. The diagnostic sensitivity and specificity of the 2Ct I-ELISA were 92.9% and 94.0%, respectively. The apparent prevalence of anti-2C antibodies for random bovine samples tested by the developed assay was 23.7%. The developed ELISA will help in augmenting the sensitivity of detection if used in combination with r3AB3 I-ELISA for sero-surveillance.
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Date 2021-08-01T12:03:42Z
2021-08-01T12:03:42Z
2013-11
 
Type Research Paper
 
Identifier Mahajan S, Mohapatra JK, Pandey LK, Sharma GK, Pattnaik B. Truncated recombinant non-structural protein 2C-based indirect ELISA for FMD sero-surveillance. J Virol Methods. 2013 Nov;193(2):405-14. doi: 10.1016/j.jviromet.2013.07.003. Epub 2013 Jul 10. PMID: 23850716.
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http://krishi.icar.gov.in/jspui/handle/123456789/51833
 
Language English
 
Relation Not Available;
 
Publisher Elsevier