Effect of permeable cryoprotectants and dextran in cryopreserving semen of broiler breeder lines.
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Title |
Effect of permeable cryoprotectants and dextran in cryopreserving semen of broiler breeder lines.
Not Available |
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Creator |
Shanmugam M.
Mahapatra R.K. |
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Subject |
chicken; cryoprotectant; dextran; fertility; semen cryopreservation
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Description |
Not Available
The aim of this study was to assess permeable cryoprotectants and dextran during broiler breeder lines semen cryopreservation in two separate experiments. Semen of PB-2 broiler breeder line was cryopreserved with 8% Ethylene Glycol (EG) or 6% dimethylformamide (DMF) in Beltsville Poultry Semen Extender (BPSE). In another experiment semen of Control broiler (CB) breeder line was cryopreserved with 8% EG, 8.2% dextran 10 kDa + 9% dimethylacetamide (DMA) or 8.2% dextran 20 kDa + 9% dimethylacetamide (DMA) in Sasaki diluent (SD). Semen cryopreserved in 0.5 ml plastic straw was thawed either at 5°C for 100 sec or 37°C for 30 sec. Semen was assessed for motility, live sperm, abnormal sperm and acrosome intact sperm. Post-thaw semen was inseminated, eggs were collected and incubated for obtaining fertility and hatchability data. The cryopreserved samples had significantly (p Not Available |
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Date |
2022-11-24T08:50:39Z
2022-11-24T08:50:39Z 2022-08-01 |
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Type |
Research Paper
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Identifier |
Not Available
1678-4324 http://krishi.icar.gov.in/jspui/handle/123456789/75160 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Instituto de Tecnologia do Paraná - Tecpar
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