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Molecular phylogeny of Capripoxviruses based on major immunodominant protein (P32) reveals circulation of host specific sheep pox and goat pox viruses in small ruminants of India

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Title Molecular phylogeny of Capripoxviruses based on major immunodominant protein (P32) reveals circulation of host specific sheep pox and goat pox viruses in small ruminants of India
Not Available
 
Creator Sumana K
Yogisharadhya R
Sathish BS
Dipti M
Apsana R
Saminathan M
Basavaraj S
Reddy GBM
 
Subject Capripox virus
Goatpoxvirus
Molecular docking
Molecular phylogeny
P32 gene
Sheeppox virus
 
Description Not Available
Sheeppox and goatpox are highly contagious viral diseases of small ruminants causing severe economic losses to the livestock farmers. The disease is enzootic in Asia including India, Middle East and African countries. In the present study, a total of 28 isolates from twenty five sheeppox and goatpox disease outbreaks were phylogenetically analyzed based on P32 gene/protein along with homology modeling and docking using heparan sulfate and UDP-glucose. Three distinct lineage-specific clusters as per their host origin were recorded. Multiple sequence analysis of P32 gene revealed that genetically similar sheeppox virus (SPPV) and goatpox virus (GTPV) strains are circulating in India. Phylogenetically, Lumpy skin disease (LSDV) and SPPV had a closer genetic relationship than GTPV. Comparative sequence alignment indicated conservation of various motifs such as glycosaminoglycan (GAG), chemokine like motif (CX3C) and Asp-Glu-any other residue-Asp (D/ExD), as well as viral specific signature residues in SPPV and GTPV isolates. Structurally, P32 protein of SPPV and GTPV with mixed α helices and β sheets resembled with crystal structure of homologue vaccinia virus H3L protein. Docking studies in P32 protein of SPPV and GTPV revealed conserved binding pattern with heparan sulfate which is involved in the virus attachment and varied glycosyltransferase fold with UDP-glucose. These findings may help in development of suitable vaccines/diagnostics and therapeutics against capripoxviruses.
Not Available
 
Date 2021-04-22T04:41:06Z
2021-04-22T04:41:06Z
2020-07-22
 
Type Research Paper
 
Identifier Sumana K, Yogisharadhya R, Sathish BS, Dipti M, Apsana R, Saminathan M, Basavaraj S and Reddy GBM. (2020). Molecular phylogeny of Capripoxviruses based on major immunodominant protein (P32) reveals circulation of host specific sheep pox and goat pox viruses in small ruminants of India. Infection Genetics and Evolution. 85:104472. 1-11. doi: 10.1016/j.meegid.2020.104472.
1567-1348 (Print)
1567-7257 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/46599
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Publishers private limited