Evaluation of recombinant BgSA3 protein based indirect-ELISA for sero-diagnosis and sero-surveillance of Babesia gibsoni in dogs
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Title |
Evaluation of recombinant BgSA3 protein based indirect-ELISA for sero-diagnosis and sero-surveillance of Babesia gibsoni in dogs
Not Available |
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Creator |
Lavanya KV
Puttalakshmamma GC Mohan HV Apsana R Yogisharadhya R Sathish SB Reddy GBM |
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Subject |
Babesia gibsoni
Diagnosis Indirect ELISA Recombinant BgSA3 protein Surveillance |
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Description |
Not Available
Canine babesiosis a tick-borne haemoprotozoan disease of dogs is of significance globally due to its rapid spread. A precise confirmatory diagnosis is required to curtail the rapid spread of infection. Our study described the evaluation of recombinant BgSA3 protein based indirect ELISA for sero-diagnosis and sero-surveillance of Babesia gibsoni infection in dogs. A partial BgSA3 gene segment 1921 bp of B. gibsoni encoding for recombinant truncated BgSA3 75 k Da protein devoid of predicted signal peptide 23 aa at N-terminus and transmembrane region 20 aa at C-terminus, was expressed in E. coli using a pET28a positive vector. The rBgSA3 protein purified under native conditions using Ni-NTA super flow cartridge was confirmed by SDS-PAGE and Western blotting using sera from dogs infected/uninfected with B. gibsoni, and erythrocyte lysate/plasma from infected/uninfected dogs. The rBgSA3 protein was specific only to B. gibsoni antibodies but did not react with uninfected sera. Further, rBgSA3 protein was evaluated for sero-diagnosis/sero-surveillance using Indirect-ELISA format. There was no cross reactivity to B. vogeli, E. canis, H. canis and D. repens infected dogs serum samples. The diagnostic sensitivity and specificity of rBgSA3 based I-ELISA was found to be 86.4 and 93.1 % respectively, in comparison with cytb based PCR assay. Additionally, rBgSA3-ELISA evaluated using survey serum samples n =287, detected 11.85 percentage samples as positive. In conclusion, B. gibsoni infection, an emerging disease is prevalent in the present study area and the standardized rBgSA3 protein based indirect-ELISA was found to be a specific and sensitive test for large scale sero-diagnosis and sero-surveillance of B. gibsoni infection in dog. Not Available |
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Date |
2021-04-22T07:47:06Z
2021-04-22T07:47:06Z 2020-01-17 |
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Type |
Research Paper
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Identifier |
Lavanya KV, Puttalakshmamma GC, Mohan HV, Apsana R, Yogisharadhya R, Sathish SB and Reddy GBM. (2021). Evaluation of recombinant BgSA3 protein based indirect-ELISA for sero-diagnosis and sero-surveillance of Babesia gibsoni in dogs. Veterinary Parasitology, 289(2021): 1-7.
0304-4017 http://krishi.icar.gov.in/jspui/handle/123456789/46618 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier Publishers private limited
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