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Comparative diagnosis of Mycobacterium avium subspecies paratuberculosis in the tissues of clinical and subclinical sheep of paratuberculosis endemic farm

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Title Comparative diagnosis of Mycobacterium avium subspecies paratuberculosis in the tissues of clinical and subclinical sheep of paratuberculosis endemic farm
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Creator Comparative Clinical Pathology
 
Subject Comparative diagnosis . Paratuberculosis . Sheep . Endemic farm
 
Description Not Available
Paratuberculosis (Johne’s disease) is the chronic infectious granulomatous enteritis of ruminants caused by
Mycobacterium avium subspecies paratuberculosis. Due to the lack of definite diagnostic tests, the early detection of
the disease is difficult. The present study was undertaken to assess comparative efficacy of diagnostic methods routinely
used in the identification and confirmation of Mycobacterium avium subspecies paratuberculosis (MAP) in the tissue
samples of naturally infected clinical and subclinical sheep. The ileum and mesenteric lymph node (MLN) tissues were
collected from 20 paratuberculous sheep from organized farm of Rajasthan. These animals were further classified as
paucibacillary (PB) (n = 8) or multibacillary (MB) (n = 12) on the basis of histopathological findings and mycobacterial
loads. The ileum and MLN sections from PB and MB and uninfected control groups were tested by indirect
immunoperoxidase technique (IPT), Ziehl Neelsen’s (ZN) method, bacterial culture, IS900 PCR and 251 gene PCR.
In PB sheep, IPT and ZN were positive in 87.5 and 50% of intestinal sections and 75 and 37.5% of MLN sections,
respectively. In MB sheep, IPT and ZN had equal sensitivity. The overall sensitivity of IPT was found to be superior
(95%) than ZN method (80%) in demonstration of acid-fast bacteria in intestinal tissues. On the bacterial cultural
examination of the intestinal and MLN tissues using Herrold’s egg yolk medium, MAP was isolated in eight (66.6%)
MB and in four (50%) PB sheep. Among 20 sheep, IS900 PCR detected 13 (65.0%) and 251 gene PCR detected 16
(80%) sheep as positive for MAP. In the PB sheep, the culture, ZN test and PCR sensitivity was found low in
comparison to MB sheep. The sensitivity of IPT in PB sheep was better than other tests in detecting the MAP infection,
which underlines its utility in confirmation of subclinical and clinical cases of paratuberculosis in sheep. The sensitivity
of 251 gene PCR assay was found better than IS900 gene PCR and has potential to be used as screening test for clinical
and subclinical paratuberculosis in sheep flocks.
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Date 2021-07-15T09:50:14Z
2021-07-15T09:50:14Z
2019-04-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/48222
 
Language English
 
Relation Not Available;
 
Publisher Not Available