Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks
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Title |
Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks
Not Available |
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Creator |
Chakraborty S
Veeregouda BM Chandranaik BM Rathnamma D Isloor S Venkatesha MD Leena G Veeresh H Patil SS |
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Subject |
Classical swine fever virus
Genogrouping Molecular characterization Molecular outbreaks |
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Description |
Not Available
The present study was carried out to characterize classical swine fever virus (CSFV) isolated from field outbreaks in suburban places of bengaluru, India, using molecular techniques and subsequent genogrouping of the virus. Various tissue samples from CSFV affected pigs (12) were collected and subjected to either virus isolation in PK-15 cell line, or RNA extraction. PCR amplification was carried out targeting the 5' NTR gene. Subsequent agarose gel electrophoresis yielded specific amplicons of 421 bp obtained from pooled samples of 3 pigs. The PCR purified products were sequenced and subjected to BLAST analysis and subsequently submitted to GenBank. The obtained nucleotide sequences were aligned using MegAlign programme and further subjected to analysis using MEGA 4 programme. All 3 field isolates were found to be grouped into subgroup 2.2 of group 2. Not Available |
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Date |
2021-08-17T08:44:41Z
2021-08-17T08:44:41Z 2011-04-06 |
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Type |
Research Paper
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Identifier |
Chakraborty S, Veeregouda BM, Chandranaik BM, Rathnamma D, Isloor S, Venkatesha MD, Leena G, Veeresh H and Patil SS. 2011. Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks. Indian Journal of Animal Sciences 81:21-25.
0367-8318 http://krishi.icar.gov.in/jspui/handle/123456789/57566 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Journal of Animal Sciences
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