Record Details

Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks
Not Available
 
Creator Chakraborty S
Veeregouda BM
Chandranaik BM
Rathnamma D
Isloor S
Venkatesha MD
Leena G
Veeresh H
Patil SS
 
Subject Classical swine fever virus
Genogrouping
Molecular characterization
Molecular outbreaks
 
Description Not Available
The present study was carried out to characterize classical swine fever virus (CSFV) isolated from field outbreaks in suburban places of bengaluru, India, using molecular techniques and subsequent genogrouping of the virus. Various tissue samples from CSFV affected pigs (12) were collected and subjected to either virus isolation in PK-15 cell line, or RNA extraction. PCR amplification was carried out targeting the 5' NTR gene. Subsequent agarose gel electrophoresis yielded specific amplicons of 421 bp obtained from pooled samples of 3 pigs. The PCR purified products were sequenced and subjected to BLAST analysis and subsequently submitted to GenBank. The obtained nucleotide sequences were aligned using MegAlign programme and further subjected to analysis using MEGA 4 programme. All 3 field isolates were found to be grouped into subgroup 2.2 of group 2.
Not Available
 
Date 2021-08-17T08:44:41Z
2021-08-17T08:44:41Z
2011-04-06
 
Type Research Paper
 
Identifier Chakraborty S, Veeregouda BM, Chandranaik BM, Rathnamma D, Isloor S, Venkatesha MD, Leena G, Veeresh H and Patil SS. 2011. Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks. Indian Journal of Animal Sciences 81:21-25.
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/57566
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences