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Multiplex PCR for the detection of five important Staphylococcus sp. in bovine subclinical mastitis milk

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Title Multiplex PCR for the detection of five important Staphylococcus sp. in bovine subclinical mastitis milk
Not Available
 
Creator Shome BR
Das Mitra S
Bhuvana M
Krithiga N
Shome R
Velu D
Prabhudas K
 
Subject Bovine mastitis
Coagulase negative staphylococcus
Diagnosis
Multiplex PCR
Staphylococcus aureus
 
Description Not Available
The multiplex PCR (mPCR) was standardized for the direct detection of 5 most significant Staphylococcus sp., viz. Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus sciuri and Staphylococcus haemolyticus from milk. Early detection and identification of predominantly Staphylococcus aureus and recent emergence of coagulase-negative staphylococci (CNS) in causing bovine mastitis is important to improve the udder health by effective treatment and control measures. The mPCR assay successfully achieved bacterial identification up to species level based on specific amplification of conserved regions of genes, viz. 23S rRNA (S. aureus), sodA (S. chromogenes and S. haemolyticus), rdr (S. epidermidis) and gap (S. sciuri) genes. The evaluation of mPCR assay with 36 ATCC reference strains and validation with 115 milk samples from subclinically infected herd and 36 bulk milk samples rendered the assay 100% specific and highly efficacious than culture method. The detection limit was found to be from 103 to 10 cfu/ml for the 5 target Staphylococcus species. The results suggest the suitability of mPCR assay to rapidly detect and differentiate 5 important Staphylococcus sp. in about 5 h. The method can be adopted for herd surveillance as a part of health management programme.
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Date 2021-08-17T08:47:51Z
2021-08-17T08:47:51Z
2012-01-10
 
Type Research Paper
 
Identifier Shome BR, Das Mitra S, Bhuvana M, Krithiga N, Shome R, Velu D and Prabhudas K. 2012. Multiplex PCR for the detection of five important Staphylococcus sp. in bovine subclinical mastitis milk. Indian Journal of Animal Sciences. 82(1):9-14.
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/57573
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences