Improvement in buffalo (Bubalus bubalis) spermatozoa functional parameters and fertility in vitro: Effect of insulin-like growth factor-I
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Title |
Improvement in buffalo (Bubalus bubalis) spermatozoa functional parameters and fertility in vitro: Effect of insulin-like growth factor-I
Not Available |
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Creator |
Selvaraju S
Nandi S Subramani T Raghavendra BS Rao SBN Ravindra JP |
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Subject |
spermatozoa
IGF-IAcrosin proteolytic activity Acrosomal reaction Mitochondrial membrane potential Sperm-zona binding Cleavage rate |
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Description |
Not Available
The aim of the current study was to assess the effect of insulin-like growth factor-I (IGF-I; 100 ng/mL) on buffalo (Bubalus Bubalis) sperm functional parameters related to in vitro fertilization. The acrosin activity (the mean diameter of halo formation in micrometers) was significantly higher in the IGF-I group (14.17 ± 1.51) compared with that in the control group (9.50 ± 0.36) at 2 h incubation. The mitochondrial membrane potential (per cent) was significantly higher in the IGF-I group compared with that in the control group at 30 min (33.27 ± 2.62 vs. 26.71 ± 1.02), 60 min (24.24 ± 3.45 vs. 18.77 ± 2.09), and 90 min (22.86 ± 3.02 vs. 16.92 ± 1.24) incubation. The percentage of spermatozoa positive for sperm nuclear chromatin decondensation (NCD) differed significantly between the groups at 90 and 120 min incubation. The comet length was significantly lower in the IGF-I group compared with that in the control group at 2 h incubation. The percentage of fragmented DNA in the tail did not differ significantly between the groups at 2 h incubation. The percentage of acrosomal-reacted spermatozoa did not differ significantly between the IGF-I and the control groups at 4 h (41.12 ± 6.44 vs. 43.53 ± 5.05) incubation. The cleavage rate (per cent) was significantly higher in the IGF-I–treated group (56.73 ± 3.70) compared with that in the control group (44.85 ± 2.15). The current study suggests that the addition of IGF-I prevents deterioration of sperm functional parameters and fertility. Not Available |
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Date |
2021-08-05T09:56:32Z
2021-08-05T09:56:32Z 2010-01-01 |
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Type |
Journal
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Identifier |
Selvaraju S, Nandi S, Subramani T, Raghavendra BS, Rao SBN and Ravindra JP (2010) Improvement in buffalo (Bubalus bubalis) spermatozoa functional parameters and fertility in vitro: Effect of insulin-like growth factor-I. Theriogenology 73:1-10
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/53290 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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