Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows
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Title |
Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows
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Creator |
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Subject |
Transition dairy cow Metritis Neutrophil molecule Myeloperoxidase CD14
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Description |
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Metritis is a postpartum uterine pathology that causes a huge economic loss due to increased culling risk and impaired milk yield and reproduction in cows. The present study was carried out to study the changes in the activity and expression of blood neutrophils in crossbred dairy cows with and without metritis. Collection of blood samples was done at 3, 2 and 1 weeks before calving, at calving and during the first day of metritis diagnosis in metritis group (n = 8) or at day 8–10 post calving in healthy group (n = 8). Neutrophils were studied for its percentage (microscopically), respiratory burst (nitro blue tetrazolium assay), myeloperoxidase (MPO) concentrations (sandwich ELISA) and expression of CXCR1, CXCR2, TLR2, TLR4, GRα, CD11b, CD14, CD25, CD44, CD47 and CD62L (RT-PCR). Immunocytochemistry was used to investigate MPO concentration and CD14 activity, and western blotting was used for estimating MPO. Although most of these parameters changed in the cows that developed metritis one week before calving, MPO and CD14 got altered much earlier. Myeloperoxidase concentrations and expression of CD14 were considerably lower starting from 2 weeks before calving in cows that developed metritis compared to healthy cows. Further studies are warranted to study the possible use of MPO and CD14 to identify transition cows more vulnerable to develop metritis several weeks before disease occurrence. Not Available |
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Date |
2021-08-09T05:31:50Z
2021-08-09T05:31:50Z 2020-11-10 |
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Type |
Research Paper
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Identifier |
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Not Available http://krishi.icar.gov.in/jspui/handle/123456789/53708 |
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Language |
English
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Relation |
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