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Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows

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Title Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows
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Creator Not Available
 
Subject Transition dairy cow Metritis Neutrophil molecule Myeloperoxidase CD14
 
Description Not Available
Metritis is a postpartum uterine pathology that causes a huge economic loss due to increased culling risk and
impaired milk yield and reproduction in cows. The present study was carried out to study the changes in the
activity and expression of blood neutrophils in crossbred dairy cows with and without metritis. Collection of
blood samples was done at 􀀀 3, 􀀀 2 and 􀀀 1 weeks before calving, at calving and during the first day of metritis
diagnosis in metritis group (n = 8) or at day 8–10 post calving in healthy group (n = 8). Neutrophils were studied
for its percentage (microscopically), respiratory burst (nitro blue tetrazolium assay), myeloperoxidase (MPO)
concentrations (sandwich ELISA) and expression of CXCR1, CXCR2, TLR2, TLR4, GRα, CD11b, CD14, CD25,
CD44, CD47 and CD62L (RT-PCR). Immunocytochemistry was used to investigate MPO concentration and CD14
activity, and western blotting was used for estimating MPO. Although most of these parameters changed in the
cows that developed metritis one week before calving, MPO and CD14 got altered much earlier. Myeloperoxidase
concentrations and expression of CD14 were considerably lower starting from 􀀀 2 weeks before calving in cows
that developed metritis compared to healthy cows. Further studies are warranted to study the possible use of
MPO and CD14 to identify transition cows more vulnerable to develop metritis several weeks before disease
occurrence.
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Date 2021-08-09T05:31:50Z
2021-08-09T05:31:50Z
2020-11-10
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/53708
 
Language English
 
Relation Not Available;
 
Publisher Not Available