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Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows

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Title Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows
 
Creator Not Available
 
Subject Transition dairy cow, metritis, neutrophil molecule, myeloperoxidase, CD14
 
Description Not Available
Metritis is a postpartum uterine pathology that causes a huge economic loss due to increased
culling risk and impaired milk yield and reproduction in cows. The present study was carried out
to study the changes in the activity and expression of blood neutrophils in crossbred dairy cows
with and without metritis. Collection of blood samples was done at -3, -2 and -1 weeks before
calving, at calving and during the first day of metritis diagnosis in metritis group (n = 8) or at day
8-10 post calving in healthy group (n = 8). Neutrophils were studied for its percentage
(microscopically), respiratory burst (nitro blue tetrazolium assay), myeloperoxidase (MPO)
concentrations (sandwich ELISA) and expression of CXCR1, CXCR2, TLR2, TLR4, GRĪ±,
CD11b, CD14, CD25, CD44, CD47 and CD62L (RT-PCR). Immunocytochemistry was used to investigate MPO concentration and CD14 activity, and western blotting was used for estimating
MPO. Although most of these parameters changed in the cows that developed metritis one week
before calving, MPO and CD14 got altered much earlier. Myeloperoxidase concentrations and
expression of CD14 were considerably lower starting from -2 weeks before calving in cows that
developed metritis compared to healthy cows. Further studies are warranted to study the possible
use of MPO and CD14 to identify transition cows more vulnerable to develop metritis several
weeks before disease occurrence.
Not Available
 
Date 2021-08-09T05:32:05Z
2021-08-09T05:32:05Z
2020-11-06
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/53710
 
Language English
 
Relation Not Available;
 
Publisher Not Available