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A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows

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Title A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows
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Subject Embryonic mortality Pregnancy Blood neutrophils Cytokines CD-Molecules
 
Description Not Available
Since long embryonic mortality has remained an area of concern affecting the reproduction, production,
and profitability of dairy cows.We investigated the possible interaction between interleukins, hormones,
and neutrophil associated CD markers during the implantation window in Karan Fries (KF) cows naturally
coming to heat. Blood collection was done on days 0 i.e. day of Artificial Insemination (AI), 10, 18, 21,
30 and on day 40 post-AI. Total leucocyte count (TLC) and neutrophil to lymphocyte (N:L) ratio were
recorded. Blood neutrophils were isolated and their number, phagocytic activity (PA), myeloperoxidase
(MPO) concentration and relative mRNA expression of cell adhesion molecules (CD-11b, CD-31, CD-44,
CD-62L) as well as progesterone-inducing-blocking-factor (PIBF) and glucocorticoid receptor alpha (GRa)
were examined. Plasma progesterone, cortisol, IL-2, IL-8, IL-6, and IL-10 were also measured. Pregnancy
was confirmed by non-return to heat, ultrasonography and per rectal examination along with progesterone
assay. Cows were further divided into pregnant (P), early embryonic mortality (EEM) and late
embryonic mortality (LEM) groups. Embryonic losses cows showed lower plasma concentration of IL-10
(500 pg/ml). Also, a 4 fold increase in the relative
mRNA expression of CD-11b and 2.5 fold changes in CD-44 expression were observed in embryonic
mortality.We observed a 1.5 fold increase in the relative mRNA expression of PIBF and a 0.5 fold increase
in GRa expression in pregnant cows compared to EEM (on day 21) and LEM (on days 30 and 40) cows.
Our results depicted that the hyperimmune status of the dam which could be due to multifactorial events
that led to the pregnancy failure. The above basic values may be used for checking the immune status
and thus timely management strategies can be taken to prevent embryonic losses.
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Date 2021-08-09T05:32:45Z
2021-08-09T05:32:45Z
2020-07-24
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/53713
 
Language English
 
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Publisher Not Available