Record Details

A Comparative Study on Changes in Total and Differential Milk Cell Counts, Activity, and Expression of Milk Phagocytes of Healthy and Mastitic Indigenous Sahiwal Cows

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title A Comparative Study on Changes in Total and Differential Milk Cell Counts, Activity, and Expression of Milk Phagocytes of Healthy and Mastitic Indigenous Sahiwal Cows
Not Available
 
Creator Not Available
 
Subject mammary gland, SCC, DLC, macrophage, neutrophil, NETs
 
Description Not Available
Milk somatic cell counts (SCCs) have been used as a gold standard to monitor mammary
health as well as an indicator of raw milk quality. The present work was undertaken
to compare the changes in the milk SCC, milk differential leukocyte counts (DLCs),
phagocytic activity (PA) of milk neutrophils and macrophages (by nitroblue tetrazolium
assay), extracellular trap formation (PicoGreen assay) and mRNA expression of various
genes in milk neutrophils and macrophages (reverse transcription–polymerase chain
reaction), and milk plasma cortisol concentration (enzyme-linked immunosorbent assay)
in healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cows. Milk was collected
from healthy, SCM, and CM cows grouped based on their SCCs and California mastitis
test with eight cows in each group. Milk SCC was estimated by SCC counter, and DLC
was done after staining the milk slide under a microscope at 100×. Total SCCs in healthy,
SCM, and CM cows were on an average of 128.30, 300.3, and 694.40 × 103 cells/mL,
respectively. Milk DLCs indicated a lower percentage of macrophage and lymphocytes
and a higher (p < 0.05) percentage of neutrophils in SCM and CM compared to healthy
milk. The percentage of mature segmented neutrophils was lower, whereas immature
band neutrophils were higher (p < 0.05) in the SCM and CM groups as compared to
healthy cows. The viability, in vitro PA, and extracellular trap formation of neutrophils
were lower (p < 0.05) in SCM and CM milk samples as compared to healthy samples.
However, the PA of macrophage remained unchanged in all the studied groups. The
relative mRNA expression of Toll-like receptors (TLR2, TLR4), myeloperoxidase, and
interleukin 2a (IL-2a) receptor (CD25) were minimum in healthy samples and increased
(p < 0.05) with the progress of mammary inflammation. However, CD44 decreased
(p < 0.05), and CD62L remained unchanged in mastitis as compared to healthy cows.
Plasma cortisol concentrations were higher (p < 0.05) in mastitis as compared to healthy
cows and were negatively correlated with the number of milk macrophages and the
functions of milk phagocytes. Estimation of total SCC, milk DLC, and activity of milk
phagocytes is essential for effective control and prevention of incidence of mastitis in
dairy cows.
Not Available
 
Date 2021-08-12T06:14:40Z
2021-08-12T06:14:40Z
2021-06-21
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/55233
 
Language English
 
Relation Not Available;
 
Publisher Not Available