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Molecular insights into the neutrophils activation in zebu cattle during seasonal variation

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Title Molecular insights into the neutrophils activation in zebu cattle during seasonal variation
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Creator Not Available
 
Subject Immunolocalization; summer season; neutrophils’ functional competence; tyrosine phosphorylation; tyrosine-phosphorylated proteins
 
Description Not Available
Earlier, we reported the impact of season on neutrophils’ functional
competence and also hypothesized that it could be the impact of
different seasons on neutrophils’ activation. In cerebration, the
present study aimed to provide insights into neutrophils’ activation
in terms of phosphorylation of tyrosine containing proteins during
different seasons. Ten Hariana cows participated in the study and
eight times blood samples were collected from each animal (twice in
each month) during each season. Phosphorylation of tyrosine proteins
was evaluated using western blotting and immunolocalization using
a fluorescent microscope. Immuno blotting identified six tyrosinephophorylated
proteins p28, p42, p44, p58, p84, and p104 in winter
and rainy seasons, whereas, p84 protein was absent in summer
season. Immunolocalization revealed positive immune reactivity
(IR) for tyrosine-phosphorylated proteins and significantly (p < 0.05)
lower percent of neutrophils showed positive IR during the summer
season as compared to winter and rainy seasons. The results of the
study evidently indicate the activation of neutrophils is mediated
through tyrosine phosphorylation and this may be a probable reason
behind the decreased neutrophils’ functional competence during
the summer. Further studies are warranted to decipher the possible
association between tyrosine phosphorylation and expression of
surface receptors required for the recruitment of neutrophils.
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Date 2021-08-26T08:17:49Z
2021-08-26T08:17:49Z
2017-02-09
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/60662
 
Language English
 
Relation Not Available;
 
Publisher Not Available