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Lactobacillus fermentum (MTCC-5898) alleviates Escherichia coli-induced inflammatory responses in intestinal epithelial cells by modulating immune genes and NF-κB signalling

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Title Lactobacillus fermentum (MTCC-5898) alleviates Escherichia coli-induced inflammatory responses in intestinal epithelial cells by modulating immune genes and NF-κB signalling
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Creator Gupta,T., Kaur,H., Kapila,S.and R. Kapila
 
Subject Lactobacillus fermentum
Inflammatory responses
Immune genes
 
Description Not Available
Aim
Dietary intervention using probiotic bacteria has emerged as a promising preventive strategy in addressing foodborne infections or gastrointestinal disorders. This study investigated the immunomodulatory effects of Lactobacillus fermentum (MTCC-5898) on Escherichia coli-induced inflammatory responses in intestinal epithelial cells.

Methods and Results
The immune response of intestinal cells (Caco-2) in the presence of probiotic Lact. fermentum was determined during exclusion, competition and displacement of E. coli as the inflammatory agent. To achieve this objective, transcriptional modulation of key immune-related genes (cytokines, pattern recognition receptors and NF-κB), release of cytokines and nuclear translocation of the NF-κB subunit p-65 were studied. Expression of pro-inflammatory cytokines IL-8, TNF-α, IFN-ϒ and IL-23 was high in E. coli-exposed intestinal cells. However, overexpression of these E. coli-induced pro-inflammatory cytokines was prevented by Lact. fermentum during exclusion and competition assays. It also modulated the transcriptional expression of regulatory cytokines (IL-10 and TGF-β), pattern recognition receptors (TLR-2 and TLR-4) and genes associated with master inflammatory regulators (NF-κB and SIGIRR) to reduce E. coli-induced inflammation. The protective effect of Lact. fermentum was further confirmed by suppression of nuclear translocation of cytoplasmic NF-κB subunit (p-65).

Conclusion
Lactobacillus fermentum alleviated E. coli-induced inflammatory responses by modulating the NF-κB signalling besides pro-inflammatory and regulatory cytokines expression.

Significance and Impact of the Study
Lactobacillus fermentum holds significant promise as a potent nutraceutical that prevents and manages inflammatory gut-associated dysfunctions.
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Date 2021-08-12T06:16:58Z
2021-08-12T06:16:58Z
2021
 
Type Research Paper
 
Identifier Gupta,T., Kaur,H., , Kapila,S. and Kapila,R. (2021) Lactobacillus fermentum (MTCC-5898) alleviates Escherichia coli induced inflammatory responses in intestinal epithelial cells by modulating immune genes and NF-κβ signalling. Journal of Applied Microbiology 10.1111/jam.15153
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http://krishi.icar.gov.in/jspui/handle/123456789/55243
 
Language English
 
Relation Not Available;
 
Publisher Wiley