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Infradian rhythmicity in milk leukocyte activity together with plasma cortisol and prolaction levels throughout the lactation period in high-yielding crossbred cows

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Title Infradian rhythmicity in milk leukocyte activity together with plasma cortisol and prolaction levels throughout the lactation period in high-yielding crossbred cows
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Subject infradian rhythm; milk leukocytes; cortisol; prolactin; crossbred cows; lactation cycle
 
Description Not Available
Infradian rhythmicity in milk leukocyte activity together with plasma cortisol and
prolaction levels throughout the lactation period in high-yielding crossbred cows has
been studied in 10 high-yielding (milk production: 5000 l per lactation) Karan Fries
crossbred (Holstein Friesian × Tharparkar) cows. Milk and blood samples were collected
from all the experimental animals. Isolation of milk phagocytes (neutrophils
and macrophages) and lymphocytes were done by density gradient centrifugation. In
vitro phagocytic index of milk neutrophils and macrophages was performed by colorimetric
NBT reductive assay. Mitogen-induced milk lymphocyte blastogenic
response was estimated by colorimetric MTT (tetrazolium) assay. Total plasma cortisol
and prolactin were estimated by enzyme immune assay. Highest value of plasma
cortisol and prolactin was observed at calving which decreased significantly
(p < 0.01) on 15th day postpartum for both prolactin and cortisol. Immune activity
of milk leukocytes was highest on day 0 colostrum and decreased significantly
(p < 0.01) on 7th day postpartum. A significant (p < 0.01) rise of plasma prolactin
was observed around 135th and 225th days postpartum, whereas a peak level of
plasma cortisol was observed at 105th, 180th, and 270th days postpartum. Phagocytic
index of milk neutrophils and macrophages remains almost in a steady state during
mid-lactation period (between 100 and 200 days postpartum). A decline in increasing
trend of milk phagocytic activity was observed during late lactation. Mitogeninduced
milk lymphocyte blastogenic response was highest on day 0 colostrum
which decreased significantly (p < 0.01) on 15th day postpartum. Con A-induced
milk lymphocyte blastogenic response showed an increasing trend from 120th to
210th days postpartum. Upon correlation study, it showed that the plasma cortisol
has a negative effect on milk leukocyte activity, while prolactin has a positive effect,
though the effect is lactation stage specific.
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Date 2021-08-26T08:15:14Z
2021-08-26T08:15:14Z
2015-06-21
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/60643
 
Language English
 
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Publisher Not Available