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Neutrophil dynamics in the blood and milk of crossbred cows naturally infected with Staphylococcus aureus

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Title Neutrophil dynamics in the blood and milk of crossbred cows naturally infected with Staphylococcus aureus
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Creator Not Available
 
Subject apoptosis, CD44, mastitis, neutrophil, neutrophil extracelluar traps, Staphylococcus aureus.
 
Description Not Available
Aim: The present study was designed to evaluate the neutrophil dynamics in terms of the functional competence during
subclinical mastitis (SCM) and clinical mastitis (CM).
Materials and Methods: A total of 146 Karan fries cows were screened and were divided into three groups as control
(n=12), SCM, n=12 and CM, n=12 groups on the basis of California mastitis test scoring, bacteriological evaluation, gross
and morphological changes in milk and by counting milk somatic cell count (SCC). Both blood and milk polymorphonuclear
neutrophils (PMNs) were isolated in the study. Phagocytic activity (PA) was studied by spectrophotometrically; neutrophil
extracelluar traps (NETs) were studied by scanning electron microscopy (SEM); CD44 was quantified by flow cytometry
and apoptosis was studied by fluorescent microscopy.
Results: Significantly (p
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Date 2021-08-26T08:14:21Z
2021-08-26T08:14:21Z
2015-03-16
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/60634
 
Language English
 
Relation Not Available;
 
Publisher Not Available