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Seasonal variation in in vitro immune activity of milk leukocytes in elite and non-elite crossbred cows of Indian subtropical semi-arid climate

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Title Seasonal variation in in vitro immune activity of milk leukocytes in elite and non-elite crossbred cows of Indian subtropical semi-arid climate
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Creator Not Available
 
Subject crossbred; cows; immunity; milk; leukocytes; somatic cells
 
Description Not Available
Immunity of mammary gland in terms of in vitro activity of milk leukocytes has
been evaluated during hot-humid, summer, and winter season in elite (n = 10) and
non-elite (n = 10) crossbred cows. Milk samples were collected from all the cows
throughout the year at 15-day interval. Milk somatic cell counts (SCC) and differential
leukocyte counts (DLC) were evaluated microscopically. Milk neutrophils, macrophages,
and lymphocytes were isolated and cultured in vitro. In vitro PI of milk
neutrophils and macrophages was evaluated by colorimetric NBT (nitro-blue tetrazolium)
reductive assay. Mitogen-induced milk lymphocyte blastogenic response was
measured by colorimetric MTT (thiazolyl blue tetrazolium bromide) assay. Milk SCC
was found to be significantly (p < 0.01) higher in elite cows compared to non-elite
cows irrespective of season. There was significant (p < 0.05) increase in milk SCC
during hot-humid season compared to winter season in both the group of the cows.
There was no significant difference between group and season in terms of DLC. In
vitro phagocytic index of elite cows was significantly (p < 0.01) higher than non-elite
cows. The phagocytic index was significantly (p < 0.01) decreased in summer and
hot-humid season compared to winter season in both the group of animals. Macrophages
isolated from elite cows having significantly (p < 0.01) lower phagocytic
index than non-elite cows which significantly (p < 0.01) decreased during summer
and hot-humid season compared to winter. In vitro milk lymphocyte proliferative
response was significantly (p < 0.01) lower in elite cows. Activity of B-lymphocytes
decreased significantly (p < 0.01) during summer and hot-humid season than winter,
but activity of T-lymphocytes remains unaltered during different seasons. In conclusion,
the mammary immunity in terms of in vitro activity of milk leukocytes is compromised
during summer and hot-humid season in elite crossbred cows; therefore,
better care and management should be taken in high-yielding cows during summer
and hot-humid season to minimize intramammary infections.
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Date 2021-08-26T08:13:54Z
2021-08-26T08:13:54Z
2015-01-30
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/60630
 
Language English
 
Relation Not Available;
 
Publisher Not Available