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Development and validation of an analytical method for determination of bronopol and kathon preservative in milk.

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Title Development and validation of an analytical method for determination of bronopol and kathon preservative in milk.
Not Available
 
Creator Chilbule, A.; Singh, R.; Mann, B.; Arora, S.; Sharma, R. and Rao, P.S.
 
Subject Bronopol , Kathon , HPLC , MI, CMI
 
Description Not Available
The bronopol and kathon are chemical preser vative which prevent degradation of milk samples and
maintain authenticity in analysis. The detection is based on
HPLC–UV–Vis spectroscopy, in which C18 column
(250 mm 9 4.6 mm, 5 lm) was used for chromatographic
separations, with a mobile phase comprising 0.1% phos phoric acid in water: Methanol: 0.1% phosphoric acid in
acetonitrile (80:10:10) at a flow rate 0.8 ml/min at ambient
temperature and with the UV detection at 250 nm for
bronopol and 274 nm for kathon. The retention time of
bronopol, kathon (MI 2-methyl-4-isothiazolin-3-one) and
kathon (CMI 5-chloro-2-methyl-4-isothiazolin-3-one) was
4.52 min, 3.98 min and 6.68 min respectively with a total
run time of 10 min. The linearity of the method was sat isfactory with regression coefficient (R2
) = 0.99. The limit
of quantification was 72, 240, 390 mg L-1 for bronopol,
kathon (MI) and kathon (CMI) respectively. Five spiked
levels (62.5, 125, 250, 500 and 1000 mg L-1
) were used to
determine the recovery of bronopol, kathon (MI) and
kathon (CMI) which was found to be 95.41 ± 11.84,
95.75 ± 8.21 and 92.22 ± 14.64% respectively, with rel ative standard deviations in the range 5.9–14.6%. The
standardized analytical method was successfully used to
rapidly detect bronopol and kathon in milk samples.
Not Available
 
Date 2021-08-26T08:23:05Z
2021-08-26T08:23:05Z
2019-05-16
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/60693
 
Language English
 
Relation Not Available;
 
Publisher Not Available