Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1.
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Title |
Immobilized aptamer on gold electrode senses trace amount of aflatoxin M1.
Not Available |
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Creator |
Pandey, A.K.; Rajput, Y.S.; Sharma R. and Singh, D.
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Subject |
Electrochemical aptasensor , Aflatoxin M1 , Aptamer, Square wave voltammetry
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Description |
Not Available
An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter elec trode and reference electrode and was achieved by sequentially layering dithiodipropionic acid, streptavidin and biotinylated-tetraethylene glycol-aptamer. Immobi lization of aptamer was monitored by cyclic voltammetry. Peak current in square wave voltammogram was inversely related to logarithmic concentration of aflatoxin M1. Dynamic range of sensor was 1–105 ppt aflatoxin M1. Sensor can be regenerated by treating electrode with 10% sodium dodecyl sulfate or 40 mM tris-HCl (pH 8.0) con taining 10 mM ethylenediaminetetraacetic acid and 0.02% tween-20 Not Available |
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Date |
2021-08-26T08:33:30Z
2021-08-26T08:33:30Z 2017-11-13 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60769 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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