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Optimization of various steps for RP-HPLC determination of carotene in milk fat.

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Title Optimization of various steps for RP-HPLC determination of carotene in milk fat.
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Creator Dhankhar, J., Sharma, R. and Mann, B
 
Subject β-carotene Milk Saponification RP-HPLC Unsaponifiable
 
Description Not Available
Main objective of this study was to optimize the analytical procedures, especially extraction and
saponification method for the better analysis of β-carotene in milk fat. Saponification method
with suitable potassium hydroxide (KOH) concentration, use of antioxidant, saponification
time, and temperature was optimized to get best extraction procedure for β-carotene . Analysis
of β-carotene was carried out by using reversed phase high performance liquid chromatography
(RP-HPLC) using C18 column. Chromatographic conditions best tested for the study involves
methanol: tetrahydrofuran: water (MeOH: THF: H2
O). The effect of time temperature
combination at 45̊C/30 min with saturated ascorbic acid had a pronounced effect on the
β-carotene extraction from milk fat. Study also reveals that a concentration level of 10M KOH
was best suited for the extraction of unsaponifiable.
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Date 2021-08-26T08:33:11Z
2021-08-26T08:33:11Z
2016-08-21
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/60766
 
Language English
 
Relation Not Available;
 
Publisher Not Available