Optimization of various steps for RP-HPLC determination of carotene in milk fat.
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Title |
Optimization of various steps for RP-HPLC determination of carotene in milk fat.
Not Available |
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Creator |
Dhankhar, J., Sharma, R. and Mann, B
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Subject |
β-carotene Milk Saponification RP-HPLC Unsaponifiable
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Description |
Not Available
Main objective of this study was to optimize the analytical procedures, especially extraction and saponification method for the better analysis of β-carotene in milk fat. Saponification method with suitable potassium hydroxide (KOH) concentration, use of antioxidant, saponification time, and temperature was optimized to get best extraction procedure for β-carotene . Analysis of β-carotene was carried out by using reversed phase high performance liquid chromatography (RP-HPLC) using C18 column. Chromatographic conditions best tested for the study involves methanol: tetrahydrofuran: water (MeOH: THF: H2 O). The effect of time temperature combination at 45̊C/30 min with saturated ascorbic acid had a pronounced effect on the β-carotene extraction from milk fat. Study also reveals that a concentration level of 10M KOH was best suited for the extraction of unsaponifiable. Not Available |
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Date |
2021-08-26T08:33:11Z
2021-08-26T08:33:11Z 2016-08-21 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60766 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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