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Synthesis and application of cephalexin imprinted polymer for solid phase extraction in milk.

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Title Synthesis and application of cephalexin imprinted polymer for solid phase extraction in milk.
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Creator Lata, K.; Sharma, R.; Naik, L. Rajput, Y.S. Mann, B.
 
Subject Cephalexin Molecular imprint polymer Non-covalent imprinting Solid phase extraction Milk
 
Description Not Available
Molecular imprinted polymer (MIP) against cephalexin was synthesized by co-polymerization of func tional monomer, cross-linker, radical initiator, along with target molecule (cephalexin) in a porogenic
material. Binding of cephalexin towards prepared MIP was studied in different solvents (water, methanol,
1 M NaCl, acetone and acetonitrile) and best binding was observed in methanol. Partition coefficient and
selectivity of prepared imprint and non-imprint was also studied. Cross reactivity in terms of binding effi ciency was also assessed with other antibiotics. Chromatographic study of MIP was carried out by pack ing prepared imprint into glass column. MIP was used as matrix in solid phase extraction (SPE) for
recovery of cephalexin from spiked milk samples for further estimation by high performance liquid chro matography. No interference was observed from milk components after elution of cephalexin from MIP,
indicating selectivity and affinity of MIP. On the other hand, interference was observed in eluate obtained
from C18 SPE column.
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Date 2021-08-26T08:31:14Z
2021-08-26T08:31:14Z
2015-03-28
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/60750
 
Language English
 
Relation Not Available;
 
Publisher Not Available